Key features and details
- Rabbit polyclonal to DLG5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DLG5 antibody
See all DLG5 primary antibodies
DescriptionRabbit polyclonal to DLG5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment (His-T7-tag) corresponding to Mouse DLG5 aa 1724-1917. (Expressed in E.coli)
DSVSLAYQRVQKVDCTSLRPVLLLGPLLDVVKEMLVNEAPGKFCRCPLEV MKASQQAIERGVKDCLFVDYKRRSGHFDVTTVASIKEITEKNRHCLLDIA PHAIERLHHMHIYPIVIFIRYKSAKHIKEQRDPVYLRDKVTQRHSKEQFE TAQKIDQEYSRYFTGVVQGGALSSICTQILAMVSQEQSKVLWIP
Database link: E9Q9R9
- IHC-P: Mouse brain tissue. WB: Recombinant mouse DLG5 protein; PC-3 cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231283 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 214 kDa.|
FunctionMay play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton.
Tissue specificityHighly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 4 PDZ (DHR) domains.
Contains 1 SH3 domain.
Cellular localizationCell junction. Cell membrane. Localized at sites of cell-cell contact.
- Information by UniProt
- Discs large homolog 5 (Drosophila) antibody
- Discs large homolog 5 antibody
- Discs large protein LP DLG antibody
Anti-DLG5 antibody (ab231283) at 2 µg/ml + PC-3 (human prostate adenocarcinoma cell line) cell lysate.
Predicted band size: 214 kDa
Anti-DLG5 antibody (ab231283) at 2 µg/ml + Recombinant mouse DLG5 protein.
Predicted band size: 214 kDa
Formalin-fixed, paraffin-embedded mouse brain tissue stained for DLG5 using ab231283 at 20 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231283 has not yet been referenced specifically in any publications.