Product nameAnti-DLG5 antibody
See all DLG5 primary antibodies
DescriptionRabbit polyclonal to DLG5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 1325 and 1375 of human DLG5 (NP_004738.3).
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86783 was affinity purified using an epitope specific to DLG5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 214 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton.
Tissue specificityHighly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 4 PDZ (DHR) domains.
Contains 1 SH3 domain.
Cellular localizationCell junction. Cell membrane. Localized at sites of cell-cell contact.
- Information by UniProt
- Discs large homolog 5 (Drosophila) antibody
- Discs large homolog 5 antibody
- Discs large protein LP DLG antibody
All lanes : Anti-DLG5 antibody (ab86783) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 214 kDa
Exposure time: 3 minutes
Detection of DLG5 by Western Blot of Immunprecipitate.
ab86783, at 1 µg/ml, staining DLG5 in HeLa whole cell lysate immunoprecipitated using ab86783 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab86783 has not yet been referenced specifically in any publications.