Overview

  • Product name

    Anti-DLK-1 antibody [3A10]
    See all DLK-1 primary antibodies
  • Description

    Mouse monoclonal [3A10] to DLK-1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human DLK-1 aa 174-349.

  • Positive control

    • Recombinant DLK-1 protein; U251 cells; NIH3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab119930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function

    May have a role in neuroendocrine differentiation.
  • Tissue specificity

    Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • Sequence similarities

    Contains 6 EGF-like domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adipocyte differentiation inhibitor protein antibody
    • Brevideltin antibody
    • Delta like 1 homolog antibody
    • Delta like homolog antibody
    • Delta like protein antibody
    • Delta1 antibody
    • DLK 1 antibody
    • DLK antibody
    • DLK-1 antibody
    • DLK1 antibody
    • DLK1_HUMAN antibody
    • FA 1 antibody
    • FA1 antibody
    • Fetal antigen 1 antibody
    • pG2 antibody
    • Preadipocyte factor 1 antibody
    • Pref 1 antibody
    • Pref antibody
    • Pref1 antibody
    • Protein delta homolog 1 antibody
    • Secredeltin antibody
    • ZOG antibody
    see all

Images

  • Immunofluorescence analysis of U251 cells using ab119930 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Anti-DLK-1 antibody [3A10] (ab119930) at 1/500 dilution + Recombinant DLK-1 protein

    Predicted band size: 41 kDa

  • Flow cytometric analysis of NIH3T3 cells using ab119930 at 1/200 dilution (green) and negative control (red).
  • ELISA using ab119930.

References

This product has been referenced in:

  • Verginelli F  et al. Paragangliomas arise through an autonomous vasculo-angio-neurogenic program inhibited by imatinib. Acta Neuropathol 135:779-798 (2018). Human . Read more (PubMed: 29305721) »
  • Wang XX  et al. Novel role of SF1 in alleviating thyroid-associated ophthalmopathy through the AMPK/mTOR signaling pathway. Gene 691:132-140 (2018). Read more (PubMed: 30562606) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry that as this particular antibody has been tested externally in flow cytometry, and we have no information on which secondary as used.

We have many anti-mouse IgG antibodies that would be suitable for use in flow cytometry with this primary. I would be pleased to suggest some. In order to do so, please confirm which conjugates you would prefer (this would narrow down the search).

I look forward to hearing from you with the requested details.

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H9 cell line upon differentiation)
Specification
H9 cell line upon differentiation
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 22 2014

Question
Answer

Gracias por tu respuesta.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo ab119930. Recibiréis una confirmación automática del mismo con más detalles.

Si necesitáis cualquier información referente al pedido, no dudéis en contactarnos, referenciando el número de éste: XXXX.

Si tuvierais cualquier otro problema, o requirierais mas información / asistencia, por favor, no dudéis en volvernos a contactar.

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Answer

Thank you for calling Abcam last night.

I have spoken to my colleagues about your wish to return the 2 vials of ab21682. Since they were purchased more than 2 weeks ago, we cannot accept them as a return, I am sorry about that. I understand that these antibodies are expensive, so below is a discount that will give you 30% off the next antibody that you purchase:

Discount Code : *******
Expiration Date: ***********

If there is anything else I can help you with, please let me know.

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Answer

Thank you for your reply.

I've sent a quotation in a separate email, and please let me know if you'd like to update this at any time.

At this time, I've been unable to find an ascites isotype control that we can add to our catalog, though I hope you've been able to find one from another vendor.

Please don't hesitate to contact me if you need anything further! I'll be happy to help. Have a great day.

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Answer

Thank you for contacting us with your questions.

I've answered these questions below, but if you have any further questions please let me know and I'll be happy to help.

1) A 100 uL vial is $337 US dollars, and we charge a flat overnight shippingfee of $45 to Canada.

2) You can place your order online by signing on to your account, you can email mailto:us.orders@abcam.comwith your order information, or give us a call at 888-772-2226 to place your order over the phone. We prefer that you use whichever method is easiest for you.

3) We ship via FedEx overnight, and deliverycan take an extra day to Canada (due to customs). If this product is ordered today, it should arrive on Monday April 23.

4) If you would like to use a fluorescent-conjugated secondary antibody, we have several options that would work well with ab119930. We have ab96879 which is a goat polyclonal antibody to mouse IgG, conjuaged to DyLight 488 (green)-

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

We also have ab7003, a donkey anti-mouse IgG conjugated to PE-

https://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab7003.html

If you prefer a colorimetric detection system, we have ab6728, a rabbit anti-mouse IgG antibody conjugated to HRP-

https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-ab6728.html

Regarding the isotype control, ab119930 is a mouse IgG1 antibody provided in ascites fluid. We have several purified mouse IgG1 isotype controls (such as ab91353) however we don't have an unpurified isotype control in ascites fluid at this time. In this case, you can use a purified isotype control like ab91353 spiked into mouse serum like ab7486 (to account for other proteins in the ascites). It may be easier for you to search on http://www.biocompare.com to see if other companies are selling a mouse IgG1 isotype control in ascites fluid. I will look into adding this kind of control to our catalog, however it maybe several days before this is available for sale on our website. I will send you the catalog number when it is available.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

Question
Answer

Thank you for your reply.

I can confirm we have the following anti mouse IgG secondary antibodies conjugated to PE which are tested and guaranteed in flow cytometry. I can recommend to review the online datasheets for further information.

ab97041 Goat anti-Mouse IgG H+L (PE) secondary antibody
https://www.abcam.com/index.html?datasheet=97041 (or use the following: https://www.abcam.com/index.html?datasheet=97041).

ab99911 Goat polyclonal Secondary Antibody to Mouse IgG (PE), pre-adsorbed
https://www.abcam.com/index.html?datasheet=99911 (or use the following: https://www.abcam.com/index.html?datasheet=99911).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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