Product nameAnti-DLK-1 antibody
See all DLK-1 primary antibodies
DescriptionRabbit polyclonal to DLK-1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide corresponding to Human DLK-1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in E18.5 Mouse Muscle Wild Type Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab21682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/150. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
FunctionMay have a role in neuroendocrine differentiation.
Tissue specificityFound within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
Sequence similaritiesContains 6 EGF-like domains.
modificationsN- and O-glycosylated.
- Information by UniProt
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
Image courtesy of Human Protein Atlas
ab21682 staining DLK-1 in human bone marrow within the haemopoietic tissue and not the adipocytes. Paraffin embedded human bone marrow was incubated with ab21682 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab21682 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
All lanes : Anti-DLK-1 antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))
Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate
Lysates/proteins at 5 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immohistochemical analysis of formaldehyde-fixed paraffin-embedded human placental tissue sections, labelling DLK-1 with ab21682 at a dilution of 1/1000 incubated for 20 minutes at 25°C. Blocking was with hydrogen peroxide at 3% incubated for 10 minutes at 25°C. The secondary used was a gekko ant-rabbit polyclonal HRP conjugate used undiluted.
This product has been referenced in:
- Yang W et al. Adiponectin promotes preadipocyte differentiation via the PPAR? pathway. Mol Med Rep 17:428-435 (2018). Read more (PubMed: 29115433) »
- Suffee N et al. Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria. Proc Natl Acad Sci U S A 114:E771-E780 (2017). Read more (PubMed: 28096344) »