Recombinant Anti-DLK-1 antibody [EPR19830] (ab210471)
![Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-297894-anti-dlk-1-antibody-epr19830-western-blot.jpg "Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19830] to DLK-1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-DLK-1 antibody [EPR19830]
See all DLK-1 primary antibodies -
Description
Rabbit monoclonal [EPR19830] to DLK-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse placenta and embryo lysates. IHC-P: Mouse cerebrum and E14.5 embryo tissues; Rat cerebrum tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19830 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
|
| IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
May have a role in neuroendocrine differentiation. -
Tissue specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
Sequence similarities
Contains 6 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 13386 Mouse
- Entrez Gene: 114587 Rat
- SwissProt: Q09163 Mouse
- Unigene: 157069 Mouse
-
Alternative names
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
Images
-
Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution
Lane 1 : Mouse placenta lysate
Lane 2 : DLK-1-knockout mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-297896-anti-dlk-1-antibody-epr19830-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-297895-anti-dlk-1-antibody-epr19830-western-blot.jpg)
Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/5000 dilution
Lane 1 : Mouse placenta lysate at 0.005 µg
Lane 2 : Mouse embryo lysate at 5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-297897-anti-dlk-1-antibody-epr19830-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-297898-anti-dlk-1-antibody-epr19830-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-DLK-1 antibody [EPR19830] (ab210471)](https://www.abcam.com/ps/products/210/ab210471/Images/ab210471-6-benefits-of-recombinant-antibodies.png)
Anti-DLK-1 antibody [EPR19830] (ab210471)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (3)
ab210471 has been referenced in 3 publications.
- Tian G et al. CpG site hypomethylation at ETS1-binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Mol Med Rep 25:N/A (2022). IHC-P ; Human . PubMed: 35059744
- Tian P et al. MicroRNA-127 Inhibits the Progression of Melanoma by Downregulating Delta-Like Homologue 1. Biomed Res Int 2020:8523465 (2020). WB ; Human . PubMed: 32051829
- Chen Y et al. Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. Aging (Albany NY) 13:1422-1439 (2020). PubMed: 33472171
