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  1. Link

    dlk-1-antibody-epr19830-ab210471.pdf

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Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-DLK-1 antibody [EPR19830] (ab210471)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (3)

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Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
  • Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
  • Anti-DLK-1 antibody [EPR19830] (ab210471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19830] to DLK-1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-DLK-1 antibody [EPR19830]
    See all DLK-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19830] to DLK-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta and embryo lysates. IHC-P: Mouse cerebrum and E14.5 embryo tissues; Rat cerebrum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19830
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

Associated products

  • Alternative Versions

    • Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574)
  • Related Products

    • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IHC-P (2)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May have a role in neuroendocrine differentiation.
  • Tissue specificity

    Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • Sequence similarities

    Contains 6 EGF-like domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P80370 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13386 Mouse
    • Entrez Gene: 114587 Rat
    • SwissProt: Q09163 Mouse
    • Unigene: 157069 Mouse
    • Alternative names

      • Adipocyte differentiation inhibitor protein antibody
      • Brevideltin antibody
      • Delta like 1 homolog antibody
      • Delta like homolog antibody
      • Delta like protein antibody
      • Delta1 antibody
      • DLK 1 antibody
      • DLK antibody
      • DLK-1 antibody
      • DLK1 antibody
      • DLK1_HUMAN antibody
      • FA 1 antibody
      • FA1 antibody
      • Fetal antigen 1 antibody
      • pG2 antibody
      • Preadipocyte factor 1 antibody
      • Pref 1 antibody
      • Pref antibody
      • Pref1 antibody
      • Protein delta homolog 1 antibody
      • Secredeltin antibody
      • ZOG antibody
      see all

    Images

    • Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
      Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
      All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution

      Lane 1 : Mouse placenta lysate
      Lane 2 : DLK-1-knockout mouse placenta lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

      The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
      Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
      All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/5000 dilution

      Lane 1 : Mouse placenta lysate at 0.005 µg
      Lane 2 : Mouse embryo lysate at 5 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

      Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-DLK-1 antibody [EPR19830] (ab210471)
      Anti-DLK-1 antibody [EPR19830] (ab210471)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (3)

    Publishing research using ab210471? Please let us know so that we can cite the reference in this datasheet.

    ab210471 has been referenced in 3 publications.

    • Tian G  et al. CpG site hypomethylation at ETS1-binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Mol Med Rep 25:N/A (2022). IHC-P ; Human . PubMed: 35059744
    • Tian P  et al. MicroRNA-127 Inhibits the Progression of Melanoma by Downregulating Delta-Like Homologue 1. Biomed Res Int 2020:8523465 (2020). WB ; Human . PubMed: 32051829
    • Chen Y  et al. Functional characterization of DLK1/MEG3 locus on chromosome 14q32.2 reveals the differentiation of pituitary neuroendocrine tumors. Aging (Albany NY) 13:1422-1439 (2020). PubMed: 33472171

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLK-1 antibody [EPR19830]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (e18 head)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    e18 head
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Nov 06 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLK-1 antibody [EPR19830]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (e16 pituitary)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    e16 pituitary
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Nov 06 2018

    Western blot abreview for Anti-DLK-1 antibody [EPR19830]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Placenta, MEF)
    Gel Running Conditions
    Non-reduced Denaturing (8% gel, Tris/Glycine buffer)
    Loading amount
    5 µg
    Specification
    Placenta, MEF
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 09 2018

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