Western blot abreview for Anti-DLK-1 antibody [EPR19830]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Placenta, MEF)
Gel Running Conditions
Non-reduced Denaturing (8% gel, Tris/Glycine buffer)
Loading amount
5 µg
Specification
Placenta, MEF
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% PBST
Dilution
1/100

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Polyclonal Goat Anti-Rabbit IgG/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
DLK1 KO placenta and MEF
Exposure
10 second(s)
Bands
Specific: 52 kDa
Positive control
Wild type placenta and MEF

Additional data

Additional Notes
We tested the final clone for this antibody, before it was added to the abcam catalogue. Lane 1 and 2: E15.5 Placenta DLK1 +/+, Lane 3 and 4: E15.5 Placenta DLK1 -/-, Lane 5 and 6: MEF DLK1 -/+, Lane 7 and 8: MEF DLK1 +/-. DLK1 is paternally expressed imprinted gene.

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Submitted Mar 09 2018

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