Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLK-1 antibody [EPR19830]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (e16 pituitary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
e16 pituitary
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1500

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Strong cytoplasmic positivity is seen in all three layers of the developing pituitary: a subpopulation of cells in the anterior while a diffuse positivy is seen in the intermediate and posterior compartments. In adult rat, I confirm the strong positivity seen in cells of the SN and VTA, in the Abcam datasheet.

Mr. Carl Hobbs

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Submitted Nov 06 2018

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