Recombinant Anti-DLL1 antibody [1F9] - BSA and Azide free (ab255780)
![Western blot - Anti-DLL1 antibody [1F9] - BSA and Azide free (ab255780)](https://www.abcam.com/ps/products/255/ab255780/Images/ab255780-450118-anti-dll1-antibody-1f9-western-blot-mouse-lung-tissue-lysate-20-ug.jpg "Western blot - Anti-DLL1 antibody [1F9] - BSA and Azide free (ab255780)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1F9] to DLL1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-DLL1 antibody [1F9] - BSA and Azide free
See all DLL1 primary antibodies -
Description
Rat monoclonal [1F9] to DLL1 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: Mouse lung tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255780 is the carrier-free version of ab252824. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab255780 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
1F9 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab255780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 78 kDa. |
Target
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Function
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
Tissue specificity
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
Sequence similarities
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13388 Mouse
- SwissProt: Q61483 Mouse
- Unigene: 4875 Mouse
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Alternative names
- Delta (Drosophila) like 1 antibody
- Delta antibody
- Delta like 1 (Drosophila) antibody
see all
Images
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Western blot - Anti-DLL1 antibody [1F9] - BSA and Azide free (ab255780)All lanes : Anti-DLL1 antibody [1F9] (ab252824) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 78 kDa
Exposure time: 5 secondsThis data was developed using ab252824, the same antibody clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17664336, 21985982).
Low expression: mouse heart (PMID: 7671806).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab255780 has not yet been referenced specifically in any publications.
