Overview

  • Product name
  • Description
    Rabbit polyclonal to DLL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    CGEEWSQDLHSSGRTDLRYS

    conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1.

  • General notes
    Dilute only prior to immediate use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/1000. Predicted molecular weight: 78 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificity
    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similarities
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all

Images

  • ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei

  • ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.

  • ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:

    Lane 1: Mouse Heart
    Lane 2: Mouse Pancreas
    Lane 3: Mouse Brain
    Lane 4: Rat Heart
    Lane 5: Rat Pancreas
    Lane 6: Rat Brain

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 1 minute
    Expected molecular weight: 77kDa
    Cell lysates at 20µg per lane.

    NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.

    Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:

    Lane 1: Mouse Heart

References

This product has been referenced in:
  • Xie J  et al. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway. Cell Transplant 26:967-982 (2017). ICC/IF ; Rat . Read more (PubMed: 28185609) »
  • Wang J  et al. EGFL7 participates in regulating biological behavior of growth hormone-secreting pituitary adenomas via Notch2/DLL3 signaling pathway. Tumour Biol 39:1010428317706203 (2017). Read more (PubMed: 28705113) »
See all 10 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your email. Ab10554 is a Fast-Track antibody - Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. The preliminary characterization of this antibody has shown the following: by Western blotting (please see image on the online datasheet) it was not possible to detect Delta 1 immunoreactivity by WB in the rat and mouse lysates tested (brain, pancreas and heart). However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. Mouse spleen has not been tested to our knowledge with this antibody. Please note that Fast-Track antibodies are released early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 1,000 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 1,000 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com Please contact us again if you have any additional questions.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Dr. Daniel Murta

Verified customer

Submitted May 15 2013

Answer


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton 20 min at RT
Blocking step
Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Adam Gracz

Verified customer

Submitted Jan 04 2013

Answer

This Rabbit polyclonal antibody to Delta 1 has been shown to work in ELISA against the peptide immunogen but has not been shown to detect the endogenous protein (WB) in the lysates specified on the datsheet. The anitbody is sold as a special investigate grade called "Fast Track" and is offered at a reduced price (EUR 215 versus EUR 330). We explain the terms and conditions associated with fast-track antibodies on our datasheet. Furthermore we offer a sustantial number of reward points (currently 1000) to researchers who get back to us with positive or negative information about the antibody. So, there is no guarantee that this antibody will work in IHC and we do not offer free trial sized samples, but we believe that it is a sufficiently novel antibody to offer to the research community under the terms described above and in further detail on the datasheet.

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Answer

Thank you for your enquiry. We cannot give you an exact time before which the signal will fade with these fluorophore conjugated secondary antibodies. The signal could last a week (the time frame that you mentioned that you would need) if your samples are prepared and stored under the correct conditions (at 4C in the dark). However, we do not know this for sure. Also, some fluorophores will fade faster than others. Please note that ab10554 has not yet been tested for application in confocal microscopy. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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