- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-DLL1 antibody
See all DLL1 primary antibodies -
DescriptionRabbit polyclonal to DLL1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide:
CGEEWSQDLHSSGRTDLRYS
conjugated to KLH, corresponding to amino acids 155-173 of Mouse Delta-1. -
General notesDilute only prior to immediate use.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. The product is sterile, filtered.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab10554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/1000. Predicted molecular weight: 78 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. |
Target
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FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
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Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
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Sequence similaritiesContains 1 DSL domain.
Contains 8 EGF-like domains. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 28514 Human
- Entrez Gene: 13388 Mouse
- Entrez Gene: 84010 Rat
- Omim: 606582 Human
- SwissProt: O00548 Human
- SwissProt: Q61483 Mouse
- SwissProt: P97677 Rat
- Unigene: 379912 Human
see all -
Alternative names
- Delta (Drosophila) like 1 antibody
- Delta antibody
- Delta like 1 (Drosophila) antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)Greene M et al., PLos One, 2016 Sep 13;11(9):e0161157. Fig 1.; doi: 10.1371/journal.pone.0161157ab10554 staining DLL1 in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 4% BSA for 1 hour. Samples were incubated with primary antibody for 1 hour at room temperature. DAPI used to stain nuclei
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL1 antibody (ab10554)ab10554 staining DLL1 in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (2ug/ml) for 1.5 hours at room temperature. A peroxidase-conjugated polyclonal was used as the secondary antibody. Nuclei stained purple with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-DLL1 antibody (ab10554)ICC/IF image of ab10554 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10554, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-DLL1 antibody (ab10554)Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Lane 2: Mouse Pancreas
Lane 3: Mouse Brain
Lane 4: Rat Heart
Lane 5: Rat Pancreas
Lane 6: Rat BrainSecondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 1 minute
Expected molecular weight: 77kDa
Cell lysates at 20µ g per lane.NB: This image shows that is was not possible to detect Delta 1 immunoreactivity by WB in the lysates tested. However little is known about Delta 1 expression and it is likely to be developmentally regulated with low expression/abundance making it difficult to detect in whole tissue homogenates. In favour of Delta 1 antibody (ab10554), the staining on the WB shows very low background and little cross reactivity in whole homogenates.
Western blot using Delta 1 antibody (ab10554) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Heart
Protocols
References
This product has been referenced in:
- Xie J et al. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway. Cell Transplant 26:967-982 (2017). ICC/IF ; Rat . Read more (PubMed: 28185609) »
- Greene M et al. Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand. PLoS One 11:e0161157 (2016). ICC/IF . Read more (PubMed: 27622512) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"