Key features and details
- Rabbit polyclonal to DLL4
- Suitable for: WB
- Reacts with: Xenopus laevis
- Isotype: IgG
Product nameAnti-DLL4 antibody
See all DLL4 primary antibodies
DescriptionRabbit polyclonal to DLL4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Xenopus laevis
Synthetic peptide within Xenopus laevis DLL4 aa 250-350. The exact sequence is proprietary.
- WB: Xenopus laevis lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 74 kDa).|
FunctionPlays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesContains 1 DSL domain.
Contains 8 EGF-like domains.
DomainThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
- Information by UniProt
- AOS6 antibody
- Delta 4 antibody
- delta 4 precursor antibody
All lanes : Anti-DLL4 antibody (ab223029) at 1 µg/ml
Lane 1 : Xenopus laevis stage 12
Lane 2 : Xenopus laevis stage 28
Lane 3 : Xenopus laevis stage 31
Lane 4 : Xenopus laevis stage 34
Lane 5 : Xenopus laevis stage 37
Lane 6 : Xenopus laevis stage 39
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223029 has not yet been referenced specifically in any publications.