Anti-DLL4 antibody (ab7280)

Rabbit polyclonal DLL4 antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Mouse, Human. Cited in 60 publication(s). Independently reviewed in 8 review(s).


  • Product name
  • Description
    Rabbit polyclonal to DLL4
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 121-134 of Human Delta4.

  • Positive control
    • Recombinant human DLL4 protein (ab84081)



Our Abpromise guarantee covers the use of ab7280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/500 - 1/2500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

The levels of DLL4 in endogenous samples may be low. Abcam has not been successful in generating western blot data using endogenous samples, but can demonstrate the specificity using recombinant proteins and overexpressing cell lysates. Some customers have been successful using ab7280 for these samples - please see published references below.

ICC/IF Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.


  • Function
    Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • Tissue specificity
    Expressed in vascular endothelium.
  • Sequence similarities
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Domain
    The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • Post-translational
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • AOS6 antibody
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like canonical Notch ligand 4 antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • Dll4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • Notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all


  • All lanes : Anti-DLL4 antibody (ab7280) at 1/1000 dilution

    Lane 1 : Recombinant human DLL4 at 0.05 µg
    Lane 2 : Whole cell lysate from HEK293 over-expressing human DLL4. at 10 µg
    Lane 3 : Whole cell lysate from control HEK293 . at 10 µg

    All lanes : Peroxidase labelled anti-rabbit antibody at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    This blot was produced under denaturing, reducing conditions. After transfer, the membrane was blocked for half an hour before being incubated overnight at 4°C with rabbit polyclonal to DLL4 (ab7280; diluted 1:1000).  Antibody binding was detected using peroxidase labelled anti-rabbit antibody (diluted 1:40,000) for half an hour at room temperature and visualised using ECL development solution.

  • IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Zhang Y  et al. MicroRNA-30a as a candidate underlying sex-specific differences in neonatal hyperoxic lung injury: implications for BPD. Am J Physiol Lung Cell Mol Physiol 316:L144-L156 (2019). Read more (PubMed: 30382766) »
  • Pattanaik S  et al. Vascular Tissue Engineering Using Scaffold-Free Prevascular Endothelial-Fibroblast Constructs. Biores Open Access 8:1-15 (2019). Read more (PubMed: 30637179) »
See all 61 Publications for this product

Customer reviews and Q&As

1-10 of 26 Abreviews or Q&A


Thank you very much for your cooperation so far and sending me the additional information.

I have one last tip i would like to share with you:

I would like to suggest to either decrease the H2O2 percentage down to 0.3% and to incubate for 10 minutes, or to block the endogenous peroxidase after the primary antibody incubation to avoid possible epitope alteration.

Please let me know if my tips improved the results.

Read More
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Human Purified protein (brain cancer)
brain cancer
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Mar 20 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Mouse Tissue sections (pancreas)

Abcam user community

Verified customer

Submitted Jul 11 2014


Je peux vous confirmer que 0.2% Tween a été utilisé pour les immunoblots et que le lysat de pancréas de souris a été chauffé avant dépôt sur le gel de polyacrylamide.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - other (Lung Endothelial)
Loading amount
20 µg
Lung Endothelial
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Victoria Bridgeman

Verified customer

Submitted May 09 2013


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

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Is the result as before: a strong band at the expected molecular weight of DLL4, and another smaller band at 72 kDa?

Can you tell me if the customer has tried any other DLL4 antibodies with these samples? Also, have they considered isolating a membrane fraction from the cell lysate? That would enrich mature DLL4 in the sample and might eliminate the 72 kDa band, if it is not a membrane-bound protein like DLL4.

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Thank you for contacting us.
Both of these antibodies are shipped at 4C on blue ice packs. They are both in stock, so if you order today by 8pm within the US, you will receive them by 10am tomorrow.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 123456.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Thank you very much for your reply and your cooperation so far.

I have found your order for these two products, and would be happy to send you a replacement. Please let me know which products you have chosen from our catalog.

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1-10 of 26 Abreviews or Q&A


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