Western blot abreview for Anti-DLL4 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: Amersham-HRP sheep anti-rabbit
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: ca.70 kDa Non-specific: ca.50 kDa

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Submitted Sep 28 2012

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