Western blot abreview for Anti-DLL4 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (Lung Endothelial)
Loading amount
20 µg
Specification
Lung Endothelial
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: DAKO Polyconcal Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
25 second(s)
Bands
Specific: 74 kDa Non-specific: 145, 180, 250 kDa
Positive control
mouse lung endothelial cell lysate

Dr. Victoria Bridgeman

Verified customer

Submitted May 09 2013

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