Anti-DLST antibody [9F4BD5] (ab110306)

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Overview

  • Product name
    Anti-DLST antibody [9F4BD5]
    See all DLST primary antibodies
  • Description
    Mouse monoclonal [9F4BD5] to DLST
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Cow, Human, Pig
  • Immunogen

    Full length native protein (purified) corresponding to Pig DLST. Purified α-ketoglutarate dehydrogenase complex (KGDHC) from Porcine heart.

  • Positive control
    • Human HDFn and HL-60 cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well)
IP Use at an assay dependent concentration.

Target

  • Function
    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Pathway
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
  • Sequence similarities
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2-oxoglutarate dehydrogenase complex component E2 antibody
      • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
      • Dihydrolipoamide S succinyltransferase antibody
      • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
      • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dlst antibody
      • DLTS antibody
      • E2 antibody
      • E2K antibody
      • mitochondrial antibody
      • ODO2_HUMAN antibody
      • OGDC-E2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
      Gating Strategy: Dead cells excluded.

      See Abreview

    • Western blot - Anti-DLST antibody [9F4BD5] (ab110306)
      Western blot - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      All lanes : Anti-DLST antibody [9F4BD5] (ab110306) at 1/4000 dilution

      Lane 1 : HeLa Cell lysate
      Lane 2 : Hek293T Cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : DyLight®650-conjugated Goat anti-mouse at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 49 kDa


      Exposure time: 1 second

      See Abreview

    References

    ab110306 has not yet been referenced specifically in any publications.

    Publishing research using ab110306? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HELA and HEK-T293)
    Loading amount
    50 µg
    Specification
    HELA and HEK-T293
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    Submitted Mar 08 2013

    Flow Cytometry abreview for Anti-DLST antibody [9F4BD5 ]

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    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Preparation
    Cell harvesting/tissue preparation method: SCRAPING
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    dead cells were excluded
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    Verified customer

    Submitted Feb 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    GOAT SERUM as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    Submitted Feb 27 2013

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