Anti-DLST antibody [9F4BD5] (ab110306)

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Overview

  • Product name

    Anti-DLST antibody [9F4BD5]
    See all DLST primary antibodies

  • Description

    Mouse monoclonal [9F4BD5] to DLST

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, In-Cell ELISA, IPmore details

  • Species reactivity

    Reacts with: Cow, Human, Pig

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human HDFn and HL-60 cells

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well)
IP Use at an assay dependent concentration.

Target

  • Function

    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Pathway

    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.

  • Sequence similarities

    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2-oxoglutarate dehydrogenase complex component E2 antibody
      • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
      • Dihydrolipoamide S succinyltransferase antibody
      • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
      • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dlst antibody
      • DLTS antibody
      • E2 antibody
      • E2K antibody
      • mitochondrial antibody
      • ODO2_HUMAN antibody
      • OGDC-E2 antibody
      see all

    Images

    • Western blot - Anti-DLST antibody [9F4BD5] (ab110306)
      Western blot - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      All lanes : Anti-DLST antibody [9F4BD5] (ab110306) at 1/4000 dilution

      Lane 1 : HeLa Cell lysate
      Lane 2 : Hek293T Cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : DyLight®650-conjugated Goat anti-mouse at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 49 kDa


      Exposure time: 1 second

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
    • Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)
      Immunocytochemistry/ Immunofluorescence - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)
      Flow Cytometry - Anti-DLST antibody [9F4BD5] (ab110306)This image is courtesy of an anonymous Abreview
      ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
      Gating Strategy: Dead cells excluded.

      See Abreview

    References

    This product has been referenced in:

    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). Read more (PubMed: 29128334) »
    See 1 Publication for this product

    Publishing research using ab110306? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HELA and HEK-T293)
    Loading amount
    50 µg
    Specification
    HELA and HEK-T293
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 08 2013

    Flow Cytometry abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Preparation
    Cell harvesting/tissue preparation method: SCRAPING
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    dead cells were excluded
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DLST antibody [9F4BD5 ]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human MDM)
    Specification
    human MDM
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    GOAT SERUM as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2013

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