Overview

  • Product name

    Anti-DLST antibody [EPR13318(B)]
    See all DLST primary antibodies
  • Description

    Rabbit monoclonal [EPR13318(B)] to DLST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DLST aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P36957

  • Positive control

    • HeLa, HepG2, MCF-7 and 293T cell lysates; Human heart and liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Pathway

      Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
    • Sequence similarities

      Belongs to the 2-oxoacid dehydrogenase family.
      Contains 1 lipoyl-binding domain.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2-oxoglutarate dehydrogenase complex component E2 antibody
      • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
      • Dihydrolipoamide S succinyltransferase antibody
      • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
      • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dlst antibody
      • DLTS antibody
      • E2 antibody
      • E2K antibody
      • mitochondrial antibody
      • ODO2_HUMAN antibody
      • OGDC-E2 antibody
      see all

    Images

    • All lanes : Anti-DLST antibody [EPR13318(B)] (ab177934) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLST with ab177934 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DLST with ab177934 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 lysate or (Lane 2) 1XPBS (negative control) using ab177934 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    • Chalker J  et al. Characterization of the impact of glutaredoxin-2 (GRX2) deficiency on superoxide/hydrogen peroxide release from cardiac and liver mitochondria. Redox Biol 15:216-227 (2018). Mouse . Read more (PubMed: 29274570) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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