Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)
Key features and details
- Mouse monoclonal [Anti-Dlx3b] to DLX-3
- Suitable for: IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-DLX-3 antibody [Anti-Dlx3b] -
Description
Mouse monoclonal [Anti-Dlx3b] to DLX-3 -
Host species
Mouse -
Tested applications
Suitable for: IHC - Wholemountmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- IHC-Wholemount: 19 somite zebrafish.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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Purity
SDS-PAGE -
Purification notes
ab174436 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
Anti-Dlx3b -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC - Wholemount | (1) |
1/100.
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Notes |
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IHC - Wholemount
1/100. |
Target
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Relevance
Involved in the development of the inner ear. Expressed during gastrulation, and shortly after gastrulation is expressed by presumptive precursor cells of the olfactory placodes, overlapping subsets of cells in the auditory vesicle, cells of the median fin fold, and cells of the visceral arches and their primordia. Tissue specificity: Expressed in the developing forebrain and fins. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 30585 Zebrafish
- SwissProt: Q01702 Zebrafish
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Alternative names
- Distal-less homeobox protein 3b antibody
- DLX-3 antibody
- dlx3 antibody
- Homeobox protein Dlx3b antibody
Images
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ab174436 staining DLX-3 in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).
Datasheets and documents
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Datasheet download
References (0)
ab174436 has not yet been referenced specifically in any publications.