Key features and details
- Mouse monoclonal [Anti-Dlx3b] to DLX-3
- Suitable for: IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG1
Product nameAnti-DLX-3 antibody [Anti-Dlx3b]
DescriptionMouse monoclonal [Anti-Dlx3b] to DLX-3
Tested applicationsSuitable for: IHC - Wholemountmore details
Species reactivityReacts with: Zebrafish
Recombinant full length protein. This information is considered to be commercially sensitive.
- IHC-Wholemount: 19 somite zebrafish.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Previously labelled as Homeobox protein Dlx3b.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
Purification notesab174436 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab174436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||1/100.|
RelevanceInvolved in the development of the inner ear. Expressed during gastrulation, and shortly after gastrulation is expressed by presumptive precursor cells of the olfactory placodes, overlapping subsets of cells in the auditory vesicle, cells of the median fin fold, and cells of the visceral arches and their primordia. Tissue specificity: Expressed in the developing forebrain and fins.
- Distal-less homeobox protein 3b antibody
- DLX-3 antibody
- dlx3 antibody
- Homeobox protein Dlx3b antibody
ab174436 staining DLX-3 in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).
ab174436 has not yet been referenced specifically in any publications.