Anti-Dlx2 antibody (ab240465)

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Western blot - Anti-Dlx2 antibody (ab240465)

    Key features and details

    • Goat polyclonal to Dlx2
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Dlx2 antibody
      See all Dlx2 primary antibodies

    • Description

      Goat polyclonal to Dlx2

    • Host species

      Goat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Rat, Human
      Predicted to work with: Mouse

    • Immunogen

      Synthetic peptide corresponding to Human Dlx2 aa 279-292 (Cysteine residue). (NP_004396.1)
      Sequence:

      C-NYPWYHQTSGSASH


      Database link: Q07687
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • WB: Human cerebellum tissue lysate; Rat brain tissue lysate.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab240465 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 34 kDa.

    1 hour primary incubation is recommended for this product.

    Target

    Images

    • Western blot - Anti-Dlx2 antibody (ab240465)
      Western blot - Anti-Dlx2 antibody (ab240465)
      All lanes : Anti-Dlx2 antibody (ab240465) at 1 µg/ml

      Lane 1 : Human cerebellum tissue lysate in RIPA buffer
      Lane 2 : Rat brain tissue lysate in RIPA buffer

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 34 kDa



      Primary incubation for 1 hour.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240465? Please let us know so that we can cite the reference in this datasheet.

    ab240465 has not yet been referenced specifically in any publications.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dlx2 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Developing Brain Section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH = 6.2
    Permeabilization
    No
    Specification
    Developing Brain Section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

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    Verified customer

    Submitted Jan 27 2020

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