RecombinantRabMAb

Recombinant Anti-DLX3 antibody [EPR8762(2)] (ab178428)

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Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
  • Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
  • Anti-DLX3 antibody [EPR8762(2)] (ab178428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8762(2)] to DLX3
  • Suitable for: IP, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-DLX3 antibody [EPR8762(2)]
    See all DLX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8762(2)] to DLX3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC

  • Species reactivity

    Reacts with: Rat, Human
    Does not react with: Mouse

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DLX3 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60479

  • Positive control

    • JAR and 293T cell line lysates; Human adult and fetal skin tissue lysates; Rat skin tissue lysate.

  • General notes

    This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab178428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC.

    • Target

    • Function

      Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.

    • Involvement in disease

      Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
      Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers.

    • Sequence similarities

      Belongs to the distal-less homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • AI4 antibody
        • Distal less homeo box 3 antibody
        • DLX 3 antibody
        • Dlx3 antibody
        • DLX3 distalless homeobox 3 antibody
        • DLX3_HUMAN antibody
        • Homeobox protein DLX 3 antibody
        • Homeobox protein DLX-3 antibody
        • Homeobox protein Dlx3 antibody
        • TDO antibody
        see all

      Images

      • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)

        Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        All lanes : Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution

        Lane 1 : JAR cell line lysate
        Lane 2 : Human skin tissue lysate
        Lane 3 : Rat skin tissue lysate
        Lane 4 : 293T cell line lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody

        Developed using the ECL technique.

        Predicted band size: 32 kDa

      • Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        Anti-DLX3 antibody [EPR8762(2)] (ab178428)

      References (2)

      Publishing research using ab178428? Please let us know so that we can cite the reference in this datasheet.

      ab178428 has been referenced in 2 publications.

      • Bhattacharya S  et al. DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis. J Invest Dermatol 138:1052-1061 (2018). PubMed: 29246798
      • Palazzo E  et al. A novel DLX3-PKC integrated signaling network drives keratinocyte differentiation. Cell Death Differ 24:717-730 (2017). ChIP . PubMed: 28186503

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