Product nameAnti-DLX3 antibody [EPR8762(2)]
See all DLX3 primary antibodies
DescriptionRabbit monoclonal [EPR8762(2)] to DLX3
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC
Species reactivityReacts with: Rat, Human
Does not react with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DLX3 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O60479
- JAR and 293T cell line lysates; Human adult and fetal skin tissue lysates; Rat skin tissue lysate.
This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).|
FunctionLikely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.
Involvement in diseaseDefects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers.
Sequence similaritiesBelongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- AI4 antibody
- Distal less homeo box 3 antibody
- DLX 3 antibody
Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution
Lane 1 : JAR cell line lysate
Lane 2 : Human skin tissue lysate
Lane 3 : Rat skin tissue lysate
Lane 4 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Predicted band size: 32 kDa
This product has been referenced in:
- Bhattacharya S et al. DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis. J Invest Dermatol 138:1052-1061 (2018). Read more (PubMed: 29246798) »
- Palazzo E et al. A novel DLX3-PKC integrated signaling network drives keratinocyte differentiation. Cell Death Differ 24:717-730 (2017). ChIP . Read more (PubMed: 28186503) »