Recombinant
RabMAb

Recombinant Anti-DLX3 antibody [EPR8762(2)] (ab178428)

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Overview

  • Product name

    Anti-DLX3 antibody [EPR8762(2)]
    See all DLX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8762(2)] to DLX3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC

  • Species reactivity

    Reacts with: Rat, Human
    Does not react with: Mouse

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DLX3 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60479

  • Positive control

    • JAR and 293T cell line lysates; Human adult and fetal skin tissue lysates; Rat skin tissue lysate.

  • General notes

    This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC.

    • Target

    • Function

      Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.

    • Involvement in disease

      Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
      Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers.

    • Sequence similarities

      Belongs to the distal-less homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • AI4 antibody
        • Distal less homeo box 3 antibody
        • DLX 3 antibody
        • Dlx3 antibody
        • DLX3 distalless homeobox 3 antibody
        • DLX3_HUMAN antibody
        • Homeobox protein DLX 3 antibody
        • Homeobox protein DLX-3 antibody
        • Homeobox protein Dlx3 antibody
        • TDO antibody
        see all

      Images

      • Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        Immunoprecipitation - Anti-DLX3 antibody [EPR8762(2)] (ab178428)

        Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        Western blot - Anti-DLX3 antibody [EPR8762(2)] (ab178428)
        All lanes : Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution

        Lane 1 : JAR cell line lysate
        Lane 2 : Human skin tissue lysate
        Lane 3 : Rat skin tissue lysate
        Lane 4 : 293T cell line lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody

        Developed using the ECL technique.

        Predicted band size: 32 kDa

      References

      This product has been referenced in:

      • Bhattacharya S  et al. DLX3-Dependent STAT3 Signaling in Keratinocytes Regulates Skin Immune Homeostasis. J Invest Dermatol 138:1052-1061 (2018). Read more (PubMed: 29246798) »
      • Palazzo E  et al. A novel DLX3-PKC integrated signaling network drives keratinocyte differentiation. Cell Death Differ 24:717-730 (2017). ChIP . Read more (PubMed: 28186503) »
      See all 2 Publications for this product

      Publishing research using ab178428? Please let us know so that we can cite the reference in this datasheet.

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