Overview

  • Product name

    Anti-DLX3 antibody [NCI-R183-14]
    See all DLX3 primary antibodies
  • Description

    Rabbit monoclonal [NCI-R183-14] to DLX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human DLX3. The exact sequence is proprietary. The immunogen is from the TDO polymorphism of DLX3

  • Positive control

    • WB: Saos-2 cell transfected with mouse TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml); Saos-2 cell transfected with human TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45, 38 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis.
  • Involvement in disease

    Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
    Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers.
  • Sequence similarities

    Belongs to the distal-less homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI4 antibody
    • Distal less homeo box 3 antibody
    • DLX 3 antibody
    • Dlx3 antibody
    • DLX3 distalless homeobox 3 antibody
    • DLX3_HUMAN antibody
    • Homeobox protein DLX 3 antibody
    • Homeobox protein DLX-3 antibody
    • Homeobox protein Dlx3 antibody
    • TDO antibody
    see all

Images

  • All lanes : Anti-DLX3 antibody [NCI-R183-14] (ab211492) at 1/1000 dilution

    Lane 1 : Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2µg/ml)
    Lane 2 : Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml)
    Lane 3 : Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2µg/ml)
    Lane 4 : Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml)

    Predicted band size: 32 kDa
    Observed band size: 38,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This product only recognizes DLX3 carrying the TDO (Tricho-Dento-Osseous syndrome) frameshift mutation.

    The data was kindly provided by: Dr. Maria Morasso (NIH/NIAMS).

References

ab211492 has not yet been referenced specifically in any publications.

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