Recombinant Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9263] to Dlx4/BP1
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Dlx4/BP1 antibody [EPR9263]
See all Dlx4/BP1 primary antibodies -
Description
Rabbit monoclonal [EPR9263] to Dlx4/BP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SW480, K562 and Human placenta lysates; Human placenta and prostatic hyperplasia tissue; SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9263 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
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Notes |
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ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa. |
Target
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Function
May play a role in determining the production of hemoglobin S. May act as a repressor. -
Tissue specificity
Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver. -
Sequence similarities
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1748 Human
- Entrez Gene: 13394 Mouse
- Entrez Gene: 303469 Rat
- Omim: 601911 Human
- SwissProt: Q92988 Human
- SwissProt: P70436 Mouse
- Unigene: 591167 Human
- Unigene: 439766 Mouse
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Alternative names
- Beta protein 1 antibody
- BP1 antibody
- Distal less homeo box 7 antibody
see all
Images
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All lanes : Anti-Dlx4/BP1 antibody [EPR9263] (ab175218) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of SW480 cells labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab175218 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175218 has not yet been referenced specifically in any publications.