Recombinant Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9263] to Dlx4/BP1
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Dlx4/BP1 antibody [EPR9263]
See all Dlx4/BP1 primary antibodies -
Description
Rabbit monoclonal [EPR9263] to Dlx4/BP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
within Human Dlx4/BP1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q92988 -
Positive control
- SW480, K562 and Human placenta lysates; Human placenta and prostatic hyperplasia tissue; SW480 cells.
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General notes
This product was previously labelled as Dlx4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9263 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/100 - 1/250. | |
IP | 1/10 - 1/100. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
WB | 1/1000 - 1/5000. Predicted molecular weight: 26 kDa. |
Target
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Function
May play a role in determining the production of hemoglobin S. May act as a repressor. -
Tissue specificity
Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver. -
Sequence similarities
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1748 Human
- Entrez Gene: 13394 Mouse
- Entrez Gene: 303469 Rat
- Omim: 601911 Human
- SwissProt: Q92988 Human
- SwissProt: P70436 Mouse
- Unigene: 591167 Human
- Unigene: 439766 Mouse
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Alternative names
- Beta protein 1 antibody
- BP1 antibody
- Distal less homeo box 7 antibody
see all
Images
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All lanes : Anti-Dlx4/BP1 antibody [EPR9263] (ab175218) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of SW480 cells labeling Dlx4/BP1 with ab175218 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab175218 at 1/10 dilution.
Protocols
References (0)
ab175218 has not yet been referenced specifically in any publications.