Recombinant
RabMAb

Recombinant Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)

Submit a review Submit a question

Overview

  • Product name

    Anti-Dlx4/BP1 antibody [EPR9263]
    See all Dlx4/BP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9263] to Dlx4/BP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human Dlx4/BP1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92988

  • Positive control

    • SW480, K562 and Human placenta lysates; Human placenta and prostatic hyperplasia tissue; SW480 cells.

  • General notes

     This product was previously labelled as Dlx4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9263

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      May play a role in determining the production of hemoglobin S. May act as a repressor.

    • Tissue specificity

      Expressed in leukemia cells and placenta. Also expressed in kidney and fetal liver.

    • Sequence similarities

      Belongs to the distal-less homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • Beta protein 1 antibody
        • BP1 antibody
        • Distal less homeo box 7 antibody
        • Distal less homeo box 9 antibody
        • distal-less homeobox 4 antibody
        • DLX4 antibody
        • DLX4_HUMAN antibody
        • DLX7 antibody
        • DLX8 antibody
        • DLX9 antibody
        • Homeobox protein DLX-4 antibody
        • Homeobox protein DLX-7 antibody
        • Homeobox protein DLX-8 antibody
        • Homeobox protein DLX4 antibody
        see all

      Images

      • Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        Western blot - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        All lanes : Anti-Dlx4/BP1 antibody [EPR9263] (ab175218) at 1/1000 dilution

        Lane 1 : SW480 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Human placenta lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 26 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)

        Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)

        Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        Immunocytochemistry/ Immunofluorescence - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)

        Immunofluorescence analysis of SW480 cells labeling Dlx4/BP1 with ab175218 at 1/100 dilution.

      • Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)
        Immunoprecipitation - Anti-Dlx4/BP1 antibody [EPR9263] (ab175218)

        Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab175218 at 1/10 dilution.

      References

      ab175218 has not yet been referenced specifically in any publications.

      Publishing research using ab175218? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab175218.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com