Product nameAnti-Dlx5 antibody
See all Dlx5 primary antibodies
DescriptionRabbit polyclonal to Dlx5
Specificityab64827 detects endogenous levels of total Dlx5 protein.
Tested applicationsSuitable for: ELISA, IHC-P, WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from an internal sequence of human Dlx5.
- Human lung carcinoma tissue and extracts from HeLa, 293 and COLO205 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64827 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab64827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
- Distal less homeo box 5 antibody
- Distal less homeobox 5 antibody
- Dlx 5 antibody
All lanes : Anti-Dlx5 antibody (ab64827) at 1/500 dilution
Lane 1 : HeLa cell extract at 30 µg
Lane 2 : 293 cell extract at 30 µg
Lane 3 : COLO205 cell extract at 30 µg
Lane 4 : COLO205 cell extract at 30 µg
plus immunising peptide at 10 µg
Predicted band size: 32 kDa
Observed band size: 32 kDa
ab64827, at 1/50 dilution, staining Dlx5 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
ab64827 at 1/100 dilution staining Dlx5 in mouse nasal bone anlagen by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed, permeabilized in 0.1% prior to blocking in 20% serum for 1 hour at RT and then incubated with ab64827 for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal, diluted 1/200, was used as the secondary antibody.
This product has been referenced in:
- Nestor MW et al. Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies. Am J Physiol Cell Physiol 308:C209-19 (2015). ICC/IF ; Mouse . Read more (PubMed: 25394470) »