Key features and details
- Rabbit polyclonal to Dlx5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dlx5 antibody
See all Dlx5 primary antibodies
DescriptionRabbit polyclonal to Dlx5
Specificityab64827 detects endogenous levels of total Dlx5 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide derived from an internal sequence of human Dlx5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64827 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab64827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
- Distal less homeo box 5 antibody
- Distal less homeobox 5 antibody
- Dlx 5 antibody
All lanes : Anti-Dlx5 antibody (ab64827) at 1/500 dilution
Lane 1 : HeLa cell extract at 30 µg
Lane 2 : 293 cell extract at 30 µg
Lane 3 : COLO205 cell extract at 30 µg
Lane 4 : COLO205 cell extract at 30 µg
plus immunising peptide at 10 µg
Predicted band size: 32 kDa
Observed band size: 32 kDa
ab64827, at 1/50 dilution, staining Dlx5 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
ab64827 has been referenced in 2 publications.
- Nestor MW et al. Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies. Am J Physiol Cell Physiol 308:C209-19 (2015). ICC/IF ; Mouse . PubMed: 25394470
- Ohlemacher SK et al. Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells. Curr Protoc Stem Cell Biol 32:1H.8.1-1H.8.20 (2015). PubMed: 25640818