Key features and details
- Rabbit polyclonal to DMC1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DMC1 antibody
See all DMC1 primary antibodies
DescriptionRabbit polyclonal to DMC1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DMC1 aa 5-153.
Database link: Q14565
- WB: Raji and K562 whole cell lysates. IHC-P: Human testis tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab237733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 38 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionMay participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Sequence similaritiesBelongs to the RecA family. DMC1 subfamily.
Contains 1 HhH domain.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- Disrupted meiotic cDNA 1 homolog antibody
- Disrupted meiotic cDNA 1, yeast, homolog of antibody
- dJ199H16.1 antibody
Paraffin-embedded human testis tissue stained for DMC1 using ab237733 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-DMC1 antibody (ab237733) at 1/500 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DMC1 (green) using ab237733 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237733 has not yet been referenced specifically in any publications.