Recombinant
RabMAb

Recombinant Anti-DMC1 antibody [EPR23207-130] (ab245217)

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Overview

  • Product name

    Anti-DMC1 antibody [EPR23207-130]
    See all DMC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23207-130] to DMC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse DMC1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q61880

  • Positive control

    • WB: His-tagged mouse DMC1 recombinant protein, Mouse testis, Rat testis lysates. IHC-P: Mouse testis, Rat testis tissues. IP: Mouse testis lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

Our Abpromise guarantee covers the use of ab245217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.
IHC-P 1/4000.
IP 1/30.
IHC-Fr 1/100.

Target

  • Function

    May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.

  • Sequence similarities

    Belongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • Disrupted meiotic cDNA 1 homolog antibody
      • Disrupted meiotic cDNA 1, yeast, homolog of antibody
      • dJ199H16.1 antibody
      • DMC 1 antibody
      • DMC1 antibody
      • DMC1 dosage suppressor of mck1 homolog antibody
      • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
      • DMC1 homologue antibody
      • DMC1_HUMAN antibody
      • DMC1H antibody
      • DNA meiotic recombinase 1 antibody
      • HsLim15 antibody
      • LIM15 antibody
      • Meiotic recombination protein DMC1/LIM15 homolog antibody
      • MGC150472 antibody
      • MGC150473 antibody
      see all

    Images

    • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat testis lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22156371). 

      Negative control: Spleen (PMID: 21085687). 

      Exposure time: 26 seconds

    • Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

      Lane 1 : His-tagged mouse DMC1 recombinant protein (aa2-340)
      Lane 2 : His-tagged mouse Rad51 recombinant protein (aa2-339)

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      No cross-reactivity with mouse Rad51 is observed. 

      Exposure time: Lane 3 seconds; Lane 2:81 seconds.

    • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      DMC1 was immunoprecipitated from 0.35 mg Mouse testis lysate 10ug with ab245217 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245217 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

      Lane 1: Mouse testis lysate 10ug

      Lane 2: ab245217 IP in Mouse testis lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245217 in Mouse testis lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.

    References

    ab245217 has not yet been referenced specifically in any publications.

    Publishing research using ab245217? Please let us know so that we can cite the reference in this datasheet.

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