Recombinant Anti-DMC1 antibody [EPR23207-130] (ab245217)

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Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23207-130] to DMC1
Suitable for: WB, IHC-P, IP, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-DMC1 antibody [EPR23207-130]
See all DMC1 primary antibodies
Description

Rabbit monoclonal [EPR23207-130] to DMC1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IP, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment within Mouse DMC1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q61880
Positive control
- WB: His-tagged mouse DMC1 recombinant protein, Mouse testis, Rat testis lysates. IHC-P: Mouse testis, Rat testis tissues. IP: Mouse testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23207-130
Isotype

IgG
Research areas

Alternative Versions
- Anti-DMC1 antibody [EPR23207-130] - BSA and Azide free (ab269869)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Our Abpromise guarantee covers the use of ab245217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 38 kDa.
IHC-P		1/4000.
IP		1/30.
IHC-Fr		1/100.

Function

May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Sequence similarities

Belongs to the RecA family. DMC1 subfamily.
Contains 1 HhH domain.
Cellular localization

Nucleus. Chromosome.
Target information above from: UniProt accession Q14565 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 13404 Mouse
- Entrez Gene: 362960 Rat
- SwissProt: Q61880 Mouse
- Unigene: 2524 Mouse
- Unigene: 45495 Rat
Alternative names
- Disrupted meiotic cDNA 1 homolog antibody
- Disrupted meiotic cDNA 1, yeast, homolog of antibody
- dJ199H16.1 antibody
- DMC 1 antibody
- DMC1 antibody
- DMC1 dosage suppressor of mck1 homolog antibody
- DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
- DMC1 homologue antibody
- DMC1_HUMAN antibody
- DMC1H antibody
- DNA meiotic recombinase 1 antibody
- HsLim15 antibody
- LIM15 antibody
- Meiotic recombination protein DMC1/LIM15 homolog antibody
- MGC150472 antibody
- MGC150473 antibody
see all

Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat testis lysate
Lane 4 : Rat spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22156371).

Negative control: Spleen (PMID: 21085687).

Exposure time: 26 seconds
Western blot - Anti-DMC1 antibody [EPR23207-130] (ab245217)

All lanes : Anti-DMC1 antibody [EPR23207-130] (ab245217) at 1/1000 dilution

Lane 1 : His-tagged mouse DMC1 recombinant protein (aa2-340)
Lane 2 : His-tagged mouse Rad51 recombinant protein (aa2-339)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

No cross-reactivity with mouse Rad51 is observed.

Exposure time: Lane 3 seconds; Lane 2:81 seconds.
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling VSIG4 with ab245217 at 1/100 (6.7 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-DMC1 antibody [EPR23207-130] (ab245217)

DMC1 was immunoprecipitated from 0.35 mg Mouse testis lysate 10ug with ab245217 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245217 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse testis lysate 10ug

Lane 2: ab245217 IP in Mouse testis lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245217 in Mouse testis lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse cerebrum. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of rat testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMC1 antibody [EPR23207-130] (ab245217)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DMC1 with ab245217 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on spermatocytes of mouse testis. The section was incubated with ab245217 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Anti-DMC1 antibody [EPR23207-130] (ab245217)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab245217? Please let us know so that we can cite the reference in this datasheet.

ab245217 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-DMC1 antibody [EPR23207-130] (ab245217)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (0)

Customer reviews and Q&As