Recombinant
RabMAb

Recombinant Anti-DMGDH antibody [EPR16908] (ab198292)

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Overview

  • Product name

    Anti-DMGDH antibody [EPR16908]
    See all DMGDH primary antibodies

  • Description

    Rabbit monoclonal [EPR16908] to DMGDH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMGDH aa 850 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UI17

  • Positive control

    • Human fetal kidney, fetal liver and HepG2 cell lysates; Human liver and kidney tissues.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).

Target

  • Pathway

    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.

  • Involvement in disease

    Defects in DMGDH are the cause of DMGDH deficiency (DMGDHD) [MIM:605850]. DMGDHD is a disorder characterized by fish odor, muscle fatigue with increased serum creatine kinase. Biochemically it is characterized by an increase of N,N-dimethylglycine (DMG) in serum and urine.

  • Sequence similarities

    Belongs to the GcvT family.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • Dimethylglycine dehydrogenase antibody
      • Dimethylglycine dehydrogenase, mitochondrial antibody
      • Dmgdh antibody
      • M2GD_HUMAN antibody
      • ME2GLYDH antibody
      • mitochondrial antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
      Note: Cytoplasm staining on human kidney tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      All lanes : Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 97 kDa


      Exposure time: 2 seconds


      5% NFDM/TBST: Blocking and diluting buffer.

    • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 97 kDa


      Exposure time: 3 minutes


      5% NFDM/TBST: Blocking and diluting buffer.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
      Note: Cytoplasm staining on human liver tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)

      DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, Human fetal liver whole cell lysate, 10ug. Lane 2: IP of DMGDH from Human fetal liver whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    References

    ab198292 has not yet been referenced specifically in any publications.

    Publishing research using ab198292? Please let us know so that we can cite the reference in this datasheet.

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