RecombinantRabMAb

Recombinant Anti-DMGDH antibody [EPR16908] (ab198292)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
  • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
  • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
  • Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)
  • Anti-DMGDH antibody [EPR16908] (ab198292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16908] to DMGDH
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-DMGDH antibody [EPR16908]
    See all DMGDH primary antibodies

  • Description

    Rabbit monoclonal [EPR16908] to DMGDH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DMGDH aa 850 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UI17

  • Positive control

    • Human fetal kidney, fetal liver and HepG2 cell lysates; Human liver and kidney tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab198292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).

Target

  • Pathway

    Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.

  • Involvement in disease

    Defects in DMGDH are the cause of DMGDH deficiency (DMGDHD) [MIM:605850]. DMGDHD is a disorder characterized by fish odor, muscle fatigue with increased serum creatine kinase. Biochemically it is characterized by an increase of N,N-dimethylglycine (DMG) in serum and urine.

  • Sequence similarities

    Belongs to the GcvT family.

  • Cellular localization

    Mitochondrion.
  • Information by UniProt

  • Database links

    • Alternative names

      • Dimethylglycine dehydrogenase antibody
      • Dimethylglycine dehydrogenase, mitochondrial antibody
      • Dmgdh antibody
      • M2GD_HUMAN antibody
      • ME2GLYDH antibody
      • mitochondrial antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
      Note: Cytoplasm staining on human kidney tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      All lanes : Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 97 kDa


      Exposure time: 2 seconds


      5% NFDM/TBST: Blocking and diluting buffer.

    • Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Western blot - Anti-DMGDH antibody [EPR16908] (ab198292)
      Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 97 kDa


      Exposure time: 3 minutes


      5% NFDM/TBST: Blocking and diluting buffer.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] (ab198292)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
      Note: Cytoplasm staining on human liver tissue was observed.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)
      Immunoprecipitation - Anti-DMGDH antibody [EPR16908] (ab198292)

      DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, Human fetal liver whole cell lysate, 10ug. Lane 2: IP of DMGDH from Human fetal liver whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-DMGDH antibody [EPR16908] (ab198292)
      Anti-DMGDH antibody [EPR16908] (ab198292)

    References (0)

    Publishing research using ab198292? Please let us know so that we can cite the reference in this datasheet.

    ab198292 has not yet been referenced specifically in any publications.

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