Key features and details
- Rabbit polyclonal to DMPO Nitrone Adduct
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-DMPO Nitrone Adduct antibody
See all DMPO Nitrone Adduct primary antibodies
DescriptionRabbit polyclonal to DMPO Nitrone Adduct
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.
Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Primary antibody notesMeasurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.
Our Abpromise guarantee covers the use of ab23702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 17 kDa.|
|ELISA||1/1000 - 1/5000.|
RelevanceFor measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)
Lane 1 : Metmyoglobin at 3 µl
Lane 2 : Metmyoglobin-DMPO at 3 µl
Lane 3 : Metmyoglobin-DMPO at 5 µl
Lane 4 : Metmyoglobin-DMPO at 10 µl
Observed band size: 17 kDa why is the actual band size different from the predicted?
ab23702 was used at a concentration of 1/1000 or 1/5000, as noted at the top of the image.
ab23702 has been referenced in 6 publications.
- Proniewski B et al. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgaq*44 Mice. Front Immunol 9:938 (2018). PubMed: 29867936
- Pathak V et al. Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. PLoS One 12:e0181732 (2017). WB ; Spinacia oleracea . PubMed: 28732060
- Kale R et al. Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A 114:2988-2993 (2017). WB . PubMed: 28265052
- Gomez-Mejiba SE et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . PubMed: 19159679
- Schildknecht S et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . PubMed: 17724253
- Ramirez DC et al. Immuno-spin trapping analyses of DNA radicals. Nat Protoc 2:512-22 (2007). PubMed: 17406615