Overview

  • Product name
    Anti-DMPO Nitrone Adduct antibody
    See all DMPO Nitrone Adduct primary antibodies
  • Description
    Rabbit polyclonal to DMPO Nitrone Adduct
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.

  • General notes


    Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.

Properties

Applications

Our Abpromise guarantee covers the use of ab23702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa.
ELISA 1/1000 - 1/5000.

Target

  • Relevance
    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Images

  • All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)

    Lane 1 : Metmyoglobin at 3 µl
    Lane 2 : Metmyoglobin-DMPO at 3 µl
    Lane 3 : Metmyoglobin-DMPO at 5 µl
    Lane 4 : Metmyoglobin-DMPO at 10 µl

    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    ab23702 was used at a concentration of 1/1000 or 1/5000, as noted at the top of the image.

References

This product has been referenced in:
  • Proniewski B  et al. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgaq*44 Mice. Front Immunol 9:938 (2018). Read more (PubMed: 29867936) »
  • Pathak V  et al. Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. PLoS One 12:e0181732 (2017). WB ; Spinacia oleracea . Read more (PubMed: 28732060) »
See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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