Key features and details
- Mouse monoclonal [N1664A] to DMPO Nitrone Adduct (Biotin)
- Suitable for: IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-DMPO Nitrone Adduct antibody [N1664A] (Biotin)
See all DMPO Nitrone Adduct primary antibodies
DescriptionMouse monoclonal [N1664A] to DMPO Nitrone Adduct (Biotin)
SpecificityRecognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
Tested applicationsSuitable for: IP, ICC/IF, WBmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to DMPO Nitrone Adduct conjugated to ovalbumin. 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
- ICC/IF: RAW 264.7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab110430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
RelevanceFor measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for DMPO Nitrone Adduct using the unconjugated version of ab110430 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Mouse IgG (Green). Counterstain: DAPI (Blue) nuclear stain.
All lanes : Unconjugated version of ab110430 at 1/200 dilution
Lane 1 : 10 um hemoglobin
Lane 2 : 10 um hemoglobin + 100 uM HOCl
Lane 3 : 10 um hemoglobin + 500 uM HOCl
Lane 4 : 10 um hemoglobin + 1000 uM HOCl
Lane 5 : 10 um hemoglobin + 20 mM DMPO
Lane 6 : 10 um hemoglobin + 100 uM HOCl + 20 mM DMPO
Lane 7 : 10 um hemoglobin + 500 uM HOCl + 20 mM DMPO
Lane 8 : 10 um hemoglobin + 1000 uM HOCl + 20 mM DMPO
Lane 9 : 10 um hemoglobin + 100 mM DMPO
Lane 10 : 10 um hemoglobin + 100 uM HOCl + 100 mM DMPO
Lane 11 : 10 um hemoglobin + 500 uM HOCl + 100 mM DMPO
Lane 12 : 10 um hemoglobin + 1000 uM HOCl + 100 mM DMPO
ab110430 has been referenced in 1 publication.
- Hamasaki T et al. Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. PLoS One 12:e0171192 (2017). ICC/IF ; Human . PubMed: 28182635