Anti-DMPO Nitrone Adduct antibody [N1664A] (Biotin) (ab110430)

Overview

  • Product name
    Anti-DMPO Nitrone Adduct antibody [N1664A] (Biotin)
    See all DMPO Nitrone Adduct primary antibodies
  • Description
    Mouse monoclonal [N1664A] to DMPO Nitrone Adduct (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Specificity
    Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to DMPO Nitrone Adduct conjugated to ovalbumin. 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

  • Positive control
    • ICC/IF: RAW 264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab110430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance
    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Images

  • RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for DMPO Nitrone Adduct using the unconjugated version of ab110430 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Mouse IgG (Green). Counterstain: DAPI (Blue) nuclear stain.

  • All lanes : Unconjugated version of ab110430 at 1/200 dilution

    Lane 1 : 10 um hemoglobin
    Lane 2 : 10 um hemoglobin + 100 uM HOCl
    Lane 3 : 10 um hemoglobin + 500 uM HOCl
    Lane 4 : 10 um hemoglobin + 1000 uM HOCl
    Lane 5 : 10 um hemoglobin + 20 mM DMPO
    Lane 6 : 10 um hemoglobin + 100 uM HOCl + 20 mM DMPO
    Lane 7 : 10 um hemoglobin + 500 uM HOCl + 20 mM DMPO
    Lane 8 : 10 um hemoglobin + 1000 uM HOCl + 20 mM DMPO
    Lane 9 : 10 um hemoglobin + 100 mM DMPO
    Lane 10 : 10 um hemoglobin + 100 uM HOCl + 100 mM DMPO
    Lane 11 : 10 um hemoglobin + 500 uM HOCl + 100 mM DMPO
    Lane 12 : 10 um hemoglobin + 1000 uM HOCl + 100 mM DMPO

References

This product has been referenced in:
  • Hamasaki T  et al. Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. PLoS One 12:e0171192 (2017). ICC/IF ; Human . Read more (PubMed: 28182635) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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