Overview

  • Product name

    Anti-DMPO Nitrone Adduct antibody [N1664A] (FITC)
    See all DMPO Nitrone Adduct primary antibodies
  • Description

    Mouse monoclonal [N1664A] to DMPO Nitrone Adduct (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab110429 recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab110429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Target

  • Relevance

    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • Alternative names

    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Images

  • ab110429 staining DMPO Nitrone Adduct in Raw264.7 cells by Immunocytochemistry/ Immunofluorescence. Left panels represent cells treat with DMPO, while right panels represent those not treated. Cells were counterstained blue with DAPI. Protocol can be found here.

References

This product has been referenced in:

  • Carmo LS  et al. Expansive Vascular Remodeling and Increased Vascular Calcification Response to Cholecalciferol in a Murine Model of Obesity and Insulin Resistance. Arterioscler Thromb Vasc Biol 39:200-211 (2019). Read more (PubMed: 30580565) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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