Key features and details
- Rabbit polyclonal to DMRT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DMRT1 antibody
See all DMRT1 primary antibodies
DescriptionRabbit polyclonal to DMRT1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human DMRT1 aa 195-363.
Database link: Q9Y5R6
- WB: Mouse heart, lung and stomach tissue lysate. IHC: Human breast cancer tissue and testis tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab222895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/20 - 1/200.|
FunctionMay be required for testis development.
Tissue specificityTestis specific.
Sequence similaritiesBelongs to the DMRT family.
Contains 1 DM DNA-binding domain.
Developmental stageExpressed in the genital ridges of six-week-old male embryos. Becomes confined to the forming seminiferous tubules (possibly Sertoli cells) at seven weeks. Not detected in female embryos.
- Information by UniProt
- CT154 antibody
- DM domain expressed in testis 1 antibody
- DM domain expressed in testis protein 1 antibody
All lanes : Anti-DMRT1 antibody (ab222895) at 1/500 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse stomach tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 40 kDa why is the actual band size different from the predicted?
Paraffin-embedded human breast cancer tissue stained for DMRT1 using ab222895 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for DMRT1 using ab222895 at 1/100 dilution in immunohistochemical analysis.
ab222895 has not yet been referenced specifically in any publications.