Overview

  • Product name

  • Description

    Rabbit polyclonal to DMT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of mouse DMT1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ELISA Use at an assay dependent dilution.
ICC/IF 1/200.

Target

  • Function

    Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • Tissue specificity

    Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
  • Involvement in disease

    Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.
  • Sequence similarities

    Belongs to the NRAMP family.
  • Post-translational
    modifications

    Ubiquitinated by WWP2.
  • Cellular localization

    Endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCT 1 antibody
    • dct-1 antibody
    • DCT1 antibody
    • Divalent cation transporter 1 antibody
    • Divalent metal transporter 1 antibody
    • DMT 1 antibody
    • DMT-1 antibody
    • DMT1 antibody
    • FLJ37416 antibody
    • Natural resistance associated macrophage protein 2 antibody
    • Natural resistance-associated macrophage protein 2 antibody
    • NRAM2_HUMAN antibody
    • NRAMP 2 antibody
    • NRAMP2 antibody
    • OK/SW-cl.20 antibody
    • Slc11a2 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
    • Solute carrier family 11 member 2 antibody
    see all

Images

  • Anti-DMT1 antibody (ab55812) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • ab55812 staining cultured ED18 rat primary hippocampal neurons by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab55812 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for overnight at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue.

    See Abreview

References

This product has been referenced in:

  • Wongjaikam S  et al. Restoring the impaired cardiac calcium homeostasis and cardiac function in iron overload rats by the combined deferiprone and N-acetyl cysteine. Sci Rep 7:44460 (2017). Read more (PubMed: 28287621) »
  • Asthana A  et al. Prion protein facilitates retinal iron uptake and is cleaved at the ß-site: Implications for retinal iron homeostasis in prion disorders. Sci Rep 7:9600 (2017). Read more (PubMed: 28851903) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Ab55735 will recognize all 4 isotypes of this protein in human as will ab123085. I've noticed that the isoforms differ at the C-terminal end. The immunogens for Ab55812 and ab117544 are against the N-terminal region of DMT1 so I would expect them recognize all isoforms as well. At this time do not offer products that recognize the specific isoforms of this target. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up