Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DMT1 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tooth)
Antigen retrieval step
Other
Permeabilization
No
Specification
Tooth
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NGS 3%
Dilution
1/200

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
in addition, this antibody does'nt work in Western blotting to neither in rate tissu nor in dental epithelium from mice.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.

Dr. Sophia HOUARI

Verified customer

Submitted Apr 29 2015

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