Anti-DMXL2 antibody (ab240690)
Key features and details
- Rabbit polyclonal to DMXL2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DMXL2 antibody
See all DMXL2 primary antibodies -
Description
Rabbit polyclonal to DMXL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human DMXL2 aa 1450-1500. The exact sequence is proprietary.
Database link: Q8TDJ6 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab240690 was affinity purified using an epitope specific to DMXL2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 340 kDa.
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IP |
Use at an assay dependent concentration.
Use 20 µl/mg lysate. |
Notes |
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WB
1/1000. Predicted molecular weight: 340 kDa. |
IP
Use at an assay dependent concentration. Use 20 µl/mg lysate. |
Target
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Function
May serve as a scaffold protein for MADD and RAB3GA on synaptic vesicles. -
Sequence similarities
Contains 16 WD repeats. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 23312 Human
- Omim: 612186 Human
- SwissProt: Q8TDJ6 Human
- Unigene: 511386 Human
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Alternative names
- DmX-like protein 2 antibody
- Dmxl2 antibody
- DMXL2_HUMAN antibody
see all
Images
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All lanes : Anti-DMXL2 antibody (ab240690) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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DMXL2 immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. Western blot was performed from the immunoprecipitate using ab240690 at 1/100 dilution.
Lane 1: ab240690 used for IP at 20 µl per reaction in Jurkat whole cell lysate.
Lane 2: Control IgG iP in Jurkat whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab240690 has not yet been referenced specifically in any publications.