Key features and details
- Rabbit polyclonal to DMXL2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DMXL2 antibody
See all DMXL2 primary antibodies
DescriptionRabbit polyclonal to DMXL2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DMXL2 aa 1450-1500. The exact sequence is proprietary.
Database link: Q8TDJ6
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab240690 was affinity purified using an epitope specific to DMXL2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab240690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 340 kDa.|
|IP||Use at an assay dependent concentration.
Use 20 µl/mg lysate.
FunctionMay serve as a scaffold protein for MADD and RAB3GA on synaptic vesicles.
Sequence similaritiesContains 16 WD repeats.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane.
- Information by UniProt
- DmX-like protein 2 antibody
- Dmxl2 antibody
- DMXL2_HUMAN antibody
All lanes : Anti-DMXL2 antibody (ab240690) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
DMXL2 immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. Western blot was performed from the immunoprecipitate using ab240690 at 1/100 dilution.
Lane 1: ab240690 used for IP at 20 µl per reaction in Jurkat whole cell lysate.
Lane 2: Control IgG iP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240690 has not yet been referenced specifically in any publications.