Overview

  • Product name

    Anti-DN-7 antibody [EPR5266]
    See all DN-7 primary antibodies
  • Description

    Rabbit monoclonal [EPR5266] to DN-7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DN-7 aa 200-300.
    Database link: Q9HBM6

  • Positive control

    • 293T, MCF7, HeLa and A549 cell lysates. Human cervical carcinoma and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.
    • Sequence similarities

      Belongs to the TAF9 family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DN-7 antibody
      • DN7 antibody
      • Neuronal cell death related protein 7 antibody
      • Neuronal cell death-related protein 7 antibody
      • TAF9-like RNA polymerase II TBP associated factor, 31 kD antibody
      • Taf9b antibody
      • TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa antibody
      • TAF9B_HUMAN antibody
      • TAF9L antibody
      • TAFII31L antibody
      • TFIID-31 antibody
      • Transcription initiation factor TFIID subunit 9-like antibody
      • Transcription initiation factor TFIID subunit 9-like protein antibody
      • Transcription initiation factor TFIID subunit 9B antibody
      • Transcription-associated factor TAFII31L antibody
      see all

    Images

    • All lanes : Anti-DN-7 antibody [EPR5266] (ab133239) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 28 kDa

    • Immunohistochemical analysis of DN-7 in paraffin embedded Human cervical carcinoma tissue, using ab133239 at a 1/250 dilution.

    • Immunohistochemical analysis of DN-7 in paraffin embedded Human tonsil tissue, using ab133239 at a 1/250 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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