DNA Binding Microplate Solution (ab156917)

Overview

  • Product name
    DNA Binding Microplate Solution
  • Tested applications
    Suitable for: Nucleotide Arraymore details
  • General notes

    ab156917 DNA high Binding solution provides a highly efficient method for binding DNA on the microplate surface. This product allows the DNA to be tightly bound to the microplate surface in a quick manner without need of overnight incubations or heating microplates.

    - Binding incubation time is only 60-90 mins
    - No blocking is required
    - Wide binding range: 0.1 ng-200 ng
    - Allows binding of short DNA fragments, which can be as short as 100 bps
    - DNA bound onto plastic surfaces is stabilized sufficiently to allow storage for 6 months prior to use

     

    Example protocol:

    1. Add 100 μl of DNA high binding solution into the microplate wells and 1-8 μl of DNA solution (0.1-200 ng) for coating.
    2. Incubate for 2 hours at 37°C.
    3. Remove the solution from the wells and wash with PBS-T 2 times.
    4. Dry at room temperature for 10-20 min. The plate can now be immediately used or stored at 4°C for up to 6 months.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at room temperature.
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab156917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Nucleotide Array Use at an assay dependent concentration.

References

ab156917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
DNA Binding microplate solution used in an ELISA with DNA isolated from HEK cells. Image shows graph of dsDNA reading with and without DNA added. (incubated with TMB read at 605nM).

Abcam user community

Verified customer

Submitted Sep 13 2018

Question
Answer

It can be used for RNA binding as well.

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Question
Answer

I can confirm that the DNA binding microplate solution, ab156917, can be used to bind single nucleotides. Basically any DNA fragment less than 100 bp will bind including single nucleotides.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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