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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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DNA Damage Assay Kit (AP sites, Colorimetric) (ab211154)

  • Datasheet
  • SDS
  • Protocol Booklet
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DNA damage - AP sites - Assay Kit (Colorimetric) (ab211154)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: DNA

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    Overview

    • Product name

      DNA Damage Assay Kit (AP sites, Colorimetric)
      See all DNA damage kits
    • Detection method

      Colorimetric
    • Sample type

      DNA
    • Assay type

      Quantitative
    • Product overview

      DNA damage Assay Kit (AP sites, Colorimetric) (ab211154) provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.


      This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 105 bp.

    • Notes

      Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins.  DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases.  Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 50 tests
      10X Wash Buffer 1 x 30ml
      ARP Solution 1 x 250µl
      ARP-DNA Standard 1 x 400µl
      DNA Binding Solution 1 x 6ml
      DNA High-Binding Plate 1 unit
      Glycogen Solution 1 x 100µl
      Reduced DNA Standard 1 x 1ml
      Sodium Acetate Solution 1 x 1ml
      Stop Solution 1 x 12ml
      Streptavidin-Enzyme Conjugate 1 x 20µl
      Substrate Solution 1 x 12ml
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Nucl. Excision Repair
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Base Excision Repair
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • Other
      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • Cell Damage

    Images

    • DNA damage - AP sites - Assay Kit (Colorimetric) (ab211154)
      DNA damage - AP sites - Assay Kit (Colorimetric) (ab211154)

      Typical ARP-DNA standard calibration curve.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab211154? Please let us know so that we can cite the reference in this datasheet.

    ab211154 has been referenced in 7 publications.

    • Mesnage R  et al. Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in rats. Commun Biol 4:471 (2021). PubMed: 33854195
    • Guo S  et al. TRIB2 desensitizes ferroptosis via ßTrCP-mediated TFRC ubiquitiantion in liver cancer cells. Cell Death Discov 7:196 (2021). PubMed: 34315867
    • Zhao S  et al. Mutation in DNA Polymerase Beta Causes Spontaneous Chromosomal Instability and Inflammation-Associated Carcinogenesis in Mice. Cancers (Basel) 11:N/A (2019). PubMed: 31412651
    • Ruiz PD  et al. MacroH2A1 Regulation of Poly(ADP-Ribose) Synthesis and Stability Prevents Necrosis and Promotes DNA Repair. Mol Cell Biol 40:N/A (2019). PubMed: 31636161
    • Wang D  et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
    • Jang J  et al. SIRT1 Enhances the Survival of Human Embryonic Stem Cells by Promoting DNA Repair. Stem Cell Reports 9:629-641 (2017). PubMed: 28689995
    • Kuczynska-Wisnik D  et al. Lack of intracellular trehalose affects formation of Escherichia coli persister cells. Microbiology 161:786-96 (2015). PubMed: 25500492

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