DNA Damage Assay Kit (AP sites, Colorimetric) (ab211154)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
DNA Damage Assay Kit (AP sites, Colorimetric)
See all DNA damage kits -
Detection method
Colorimetric -
Sample type
DNA -
Assay type
Quantitative -
Product overview
DNA damage Assay Kit (AP sites, Colorimetric) (ab211154) provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.
This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 105 bp.
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Notes
Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 50 tests 10 tests 10X Wash Buffer 1 x 30ml 1 x 10ml ARP Solution 1 x 250µl 1 x 50µl ARP-DNA Standard 1 x 400µl 1 x 75µl DNA Binding Solution 1 x 6ml 1 x 1.25ml DNA High-Binding Plate 1 unit 1 unit Glycogen Solution 1 x 100µl 1 x 10µl Reduced DNA Standard 1 x 1ml 1 x 200µl Sodium Acetate Solution 1 x 1ml 1 x 200µl Stop Solution 1 x 12ml 1 x 4ml Streptavidin-Enzyme Conjugate 1 x 20µl 1 x 10µl Substrate Solution 1 x 12ml 1 x 4ml -
Research areas
Images
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DNA damage - AP sites - Assay Kit (Colorimetric) (ab211154)Typical ARP-DNA standard calibration curve.
Protocols
References
This product has been referenced in:
- Jang J et al. SIRT1 Enhances the Survival of Human Embryonic Stem Cells by Promoting DNA Repair. Stem Cell Reports 9:629-641 (2017). Read more (PubMed: 28689995) »
- Kuczynska-Wisnik D et al. Lack of intracellular trehalose affects formation of Escherichia coli persister cells. Microbiology 161:786-96 (2015). Read more (PubMed: 25500492) »
