DNA Damage Assay Kit (AP sites, Colorimetric) (ab211154)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: DNA
Overview
-
Product name
DNA Damage Assay Kit (AP sites, Colorimetric)
See all DNA damage kits -
Detection method
Colorimetric -
Sample type
DNA -
Assay type
Quantitative -
Product overview
DNA damage Assay Kit (AP sites, Colorimetric) (ab211154) provides a sensitive and specific method to monitor the formation of apurinic/apyrimidinic (AP) sites, one of the major types of DNA lesions.
This DNA damage assay uses an APR (Aldehyde Reactive Probe) that reacts specifically with an aldehyde group on the open ring form of AP sites. AP sites are then tagged with biotin residues that can later be quantified using an streptavidin-enzyme conjugate that is easily detected by absorbance at OD450 nm. The kit has a detection sensitivity range of 4-40 AP sites per 1 x 105 bp.
-
Notes
Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged lipids and proteins. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, apurinic/apyrimidinic (AP or abasic) site is one of the prevalent lesions of oxidative DNA damage. Abasic sites arise in DNA at a significant rate by spontaneous base loss as in depurination, by DNA oxidation, or by the action of DNA glycosylases. Estimates of the number of abasic sites generated per mammalian cell run as high as 50,000 to 200,000 per day. Unrepaired abasic sites inhibit topoisomerases, replication, and transcription and can be mutagenic because of bypass synthesis on nontemplated DNA.
-
Platform
Microplate reader
Properties
-
Storage instructions
Please refer to protocols. -
Components 50 tests 10X Wash Buffer 1 x 30ml ARP Solution 1 x 250µl ARP-DNA Standard 1 x 400µl DNA Binding Solution 1 x 6ml DNA High-Binding Plate 1 unit Glycogen Solution 1 x 100µl Reduced DNA Standard 1 x 1ml Sodium Acetate Solution 1 x 1ml Stop Solution 1 x 12ml Streptavidin-Enzyme Conjugate 1 x 20µl Substrate Solution 1 x 12ml -
Research areas
Images
-
DNA damage - AP sites - Assay Kit (Colorimetric) (ab211154)
Typical ARP-DNA standard calibration curve.
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab211154 has been referenced in 7 publications.
- Mesnage R et al. Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in rats. Commun Biol 4:471 (2021). PubMed: 33854195
- Guo S et al. TRIB2 desensitizes ferroptosis via ßTrCP-mediated TFRC ubiquitiantion in liver cancer cells. Cell Death Discov 7:196 (2021). PubMed: 34315867
- Zhao S et al. Mutation in DNA Polymerase Beta Causes Spontaneous Chromosomal Instability and Inflammation-Associated Carcinogenesis in Mice. Cancers (Basel) 11:N/A (2019). PubMed: 31412651
- Ruiz PD et al. MacroH2A1 Regulation of Poly(ADP-Ribose) Synthesis and Stability Prevents Necrosis and Promotes DNA Repair. Mol Cell Biol 40:N/A (2019). PubMed: 31636161
- Wang D et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
- Jang J et al. SIRT1 Enhances the Survival of Human Embryonic Stem Cells by Promoting DNA Repair. Stem Cell Reports 9:629-641 (2017). PubMed: 28689995
- Kuczynska-Wisnik D et al. Lack of intracellular trehalose affects formation of Escherichia coli persister cells. Microbiology 161:786-96 (2015). PubMed: 25500492