Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader
- Assay time: 3 hr
Product nameDNA demethylase (total) Activity Quantification Assay Kit
See all DNA demethylase kits
Assay typeEnzyme activity
Assay time3h 00m
Species reactivityReacts with: Mouse, Human
Predicted to work with: Mammals
DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.
DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.
ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 8µl Demethylase Assay Buffer 1 x 3ml 1 x 6ml Demethylase Substrate 1 x 0.1ml 1 x 0.2ml Demethylation Standard, 10 µg/mL 1 x 20µl 1 x 40µl Detecting Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 5ml 1 x 10ml Stop Solution 1 x 3ml 1 x 6ml Substrate Binding Solution 1 x 5ml 1 x 10ml
Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.
ab113472 has not yet been referenced specifically in any publications.