The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed Gyrase in cell extracts.
Primary antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
Western blot - Anti-DNA Gyrase A antibody [4D3] (ab75585)
All lanes : Anti-DNA Gyrase A antibody [4D3] (ab75585) at 1/1000 dilution
Lane 1 : B24 (a domain of the DNA Gyrase B protein) Lane 2 : B43 (a domain of the DNA Gyrase B protein) Lane 3 : B (the whole DNA Gyrase B protein) Lane 4 : A59 (a domain of the DNA Gyrase A protein) Lane 5 : A (the whole DNA Gyrase A protein)
Lysates/proteins at 200 µg per lane.
Secondary All lanes : HRP labelled rabbit anti mouse IgG at 1/5000 dilution