Product nameAnti-DNA Gyrase A antibody
DescriptionRabbit polyclonal to DNA Gyrase A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Escherichia coli, Staphylococcus aureus
Purified E. coli DNA Gyrase A protein (over expressed from E. coli)
- E.coli cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Purification notesThe antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
Our Abpromise guarantee covers the use of ab75594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
The antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceDNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
- DNA gyrase subunit A antibody
- GyrA antibody
- HisW antibody
All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution
Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg
All lanes : goat anti rabbit Igs at 1/5000 dilution
Observed band size: 97 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Aldred KJ et al. A RADAR-Based Assay to Isolate Covalent DNA Complexes in Bacteria. Antibiotics (Basel) 8:N/A (2019). Read more (PubMed: 30818799) »
- Aedo S & Tse-Dinh YC Isolation and quantitation of topoisomerase complexes accumulated on escherichia coli chromosomal DNA. Antimicrob Agents Chemother 56:5458-64 (2012). WB . Read more (PubMed: 22869559) »