Overview

  • Product name

    Anti-DNA Gyrase A antibody
  • Description

    Rabbit polyclonal to DNA Gyrase A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Escherichia coli, Staphylococcus aureus
  • Immunogen

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • Positive control

    • E.coli cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    The antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
    The antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • Database links

      • Alternative names

        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Images

      • All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution

        Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
        Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
        Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
        Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
        Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
        Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
        Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
        Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg

        Secondary
        All lanes : goat anti rabbit Igs at 1/5000 dilution

        Observed band size: 97 kDa
        why is the actual band size different from the predicted?

      References

      This product has been referenced in:

      • Aldred KJ  et al. A RADAR-Based Assay to Isolate Covalent DNA Complexes in Bacteria. Antibiotics (Basel) 8:N/A (2019). Read more (PubMed: 30818799) »
      • Aedo S & Tse-Dinh YC Isolation and quantitation of topoisomerase complexes accumulated on escherichia coli chromosomal DNA. Antimicrob Agents Chemother 56:5458-64 (2012). WB . Read more (PubMed: 22869559) »
      See all 2 Publications for this product

      Customer reviews and Q&As

      1-2 of 2 Abreviews or Q&A

      Answer

      Thank you for contacting us.

      Most of our antibodies are stable at -20C for up to five years. For short term storage we recommend storing at 4C. It is also important to aliquot them in order to minimize damage due to freezing and thawing.

      If after following these recommendations you are still experiencing problems with the antibody, please let us know, and I’ll be happy to follow this quality issue up.

      All of our products are covered by our Abpromise guarantee; which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

      Please let me know how the antibody performs, and do not hesitate to contact us if it does not do it as expected.

      Read More

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with a different lot. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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