Key features and details
- Mouse monoclonal [9G8] to DNA Gyrase B
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG2a
Product nameAnti-DNA Gyrase B antibody [9G8]
See all DNA Gyrase B primary antibodies
DescriptionMouse monoclonal [9G8] to DNA Gyrase B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Escherichia coli
Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesProduced from tissue culture supernatant and purified on a Protein A column using the low salt method.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed gyrase in cell extracts.
The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceDNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
- SwissProt: P0AES6 Escherichia coli
- acrB antibody
- DNA gyrase subunit B antibody
- himB antibody
All lanes : Anti-DNA Gyrase B antibody [9G8] (ab75593) at 1/1000 dilution
Lane 1 : B24 domain of Gyrase B protein (purified from E.coli)
Lane 2 : B43 domain of Gyrase B protein (purified from E.coli)
Lane 3 : Gyrase B whole protein (purified from E.coli)
Lane 4 : A59 domain of Gyrase A protein (purified from E.coli)
Lane 5 : Gyrase A whole protein (purified from E.coli)
Lysates/proteins at 200 µg per lane.
All lanes : HRP labelled rabbit anti mouse IgGs at 1/5000 dilution
Observed band size: 90 kDa why is the actual band size different from the predicted?
ab75593 has not yet been referenced specifically in any publications.