Product nameDNA Isolation & Bisulfite Conversion Kit
See all DNA modification kits
Sample typeSerum, Plasma, Adherent cells, Suspension cells
Assay time2h 20m
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.
Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discriminate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes and is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 40 tests 1X TE Buffer 1 x 16ml DNA Binding Buffer 1 x 14ml DNA Denature Solution 1 x 0.25ml DNA Digestion Powder 1 vial DNA Digestion Solution 1 x 1ml DNA Elution Buffer 1 x 1ml DNA Isolation Buffer 1 x 22ml DNA Isolation Enhancer 1 x 0.1ml DNA Modifier Powder 4 vials DNA Modifier Solution 1 x 5ml DNA Washing Buffer 1 x 3ml F-Collection Tube 80 units F-Spin Column 80 units
ab117124 has not yet been referenced specifically in any publications.