Overview

  • Product name

    DNA Isolation & Bisulfite Conversion Kit
    See all DNA modification kits
  • Sample type

    Serum, Plasma, Adherent cells, Suspension cells
  • Assay time

    2h 20m
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.


    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discriminate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
    DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes and is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.


    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

Properties

Protocols

References

ab117124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

25 ul serum or 50 ul of plasma is fine for using with this kit. The EBM3 buffer can be directly added into the serum/plasma sample. 25 ul of serum would be fine for obtaining modified DNA. However we are not sure if the modified DNA can be obtained from the 50 ul of plasma as in general DNA concentration in most of plasma samples is 0-10 ng/ml.

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Question
Answer

Thank you for contacting us.

Unfortunately, we do not carry DNA purification columns, but we do carry a kit:

EpiSeeker DNA purification & modification Kit (ab117124) [ https://www.abcam.com/EpiSeeker-DNA-purification--modification-Kit-ab117124.html] which might be of help dependent on the application.

I am sorry that we donot have exactly what you are looking for.
Please do not hesitate to contact us if you need any more advice or information.

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