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Customer kindly contacted us regarding the use of smaller sample sizes with this kit. They are only able to obtain 25ul serum/ 50 ul plasma samples. Would the kit be able to work with this sample size if diluted up to the 500ul recommended? What would be the suggested diluent?
Asked on Mar 15 2013
25 ul serum or 50 ul of plasma is fine for using with this kit. The EBM3 buffer can be directly added into the serum/plasma sample. 25 ul of serum would be fine for obtaining modified DNA. However we are not sure if the modified DNA can be obtained from the 50 ul of plasma as in general DNA concentration in most of plasma samples is 0-10 ng/ml.
Answered on Mar 15 2013