Anti-DNA Ligase III/LIG3 antibody (ab97404)
Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies -
Description
Rabbit polyclonal to DNA Ligase III/LIG3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 116-403.
Database link: NP_039269 -
Positive control
- HepG2 whole cell lysate; A549 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
|
|
IHC-P |
1/100 - 1/250.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 113 kDa. |
IHC-P
1/100 - 1/250. |
Target
-
Function
Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
Tissue specificity
Testis, thymus, prostate and heart. -
Sequence similarities
Belongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 514719 Cow
- Entrez Gene: 3980 Human
- Entrez Gene: 16882 Mouse
- Entrez Gene: 303369 Rat
- Omim: 600940 Human
- SwissProt: P49916 Human
- SwissProt: P97386 Mouse
- Unigene: 100299 Human
see all -
Alternative names
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
see all
Images
-
Anti-DNA Ligase III/LIG3 antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 113 kDa7.5% SDS-PAGE
Secondary antibody - anti-rabbit HRP (ab6721)
-
ab97404, at 1/100 dilution, staining DNA Ligase III/LIG3 in paraffin-embedded A549 xenograft by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab97404 has not yet been referenced specifically in any publications.