Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies
DescriptionRabbit polyclonal to DNA Ligase III/LIG3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 (C terminal).
Database link: P49916
- Extracts of testis cell lines.
This product was previously labelled as DNA Ligase III
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionInteracts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Tissue specificityTestis, thymus, prostate and heart.
Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger.
- Information by UniProt
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
Anti-DNA Ligase III/LIG3 antibody (ab185815) at 1/500 dilution + extracts of testis cell lines
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling DNA Ligase III/LIG3 with ab185815 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling DNA Ligase III/LIG3 with ab185815 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab185815. Blue DAPI for nuclear staining.
ab185815 has been referenced in 1 publication.
- Galanos P et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol 19:37 (2018). PubMed: 29548335