Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies
DescriptionRabbit polyclonal to DNA Ligase III/LIG3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide within Human DNA Ligase III/LIG3 aa 800-850. The exact sequence is proprietary. (CAA59230.1).
Database link: P49916-3
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
This product was previously labelled as DNA Ligase III
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224820 was affinity purified using an epitope specific to DNA Ligase III/LIG3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 103 kDa.|
FunctionInteracts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Tissue specificityTestis, thymus, prostate and heart.
Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger.
- Information by UniProt
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
All lanes : Anti-DNA Ligase III/LIG3 antibody (ab224820) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
DNA Ligase III/LIG3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224820 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224820 at 1 µg/ml.
Lane 1: ab224820 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224820 has not yet been referenced specifically in any publications.