Overview

  • Product name

    Anti-DNA Ligase III/LIG3 antibody
    See all DNA Ligase III/LIG3 primary antibodies
  • Description

    Rabbit polyclonal to DNA Ligase III/LIG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human DNA Ligase III/LIG3 aa 800-850. The exact sequence is proprietary. (CAA59230.1).
    Database link: P49916-3

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as DNA Ligase III

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab224820 was affinity purified using an epitope specific to DNA Ligase III/LIG3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.

Target

  • Function

    Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Tissue specificity

    Testis, thymus, prostate and heart.
  • Sequence similarities

    Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

Images

  • All lanes : Anti-DNA Ligase III/LIG3 antibody (ab224820) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 103 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

  • DNA Ligase III/LIG3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224820 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224820 at 1 µg/ml.

    Lane 1: ab224820 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab224820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224820.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up