Product nameAnti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies
DescriptionRabbit polyclonal to DNA Ligase III/LIG3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide within Human DNA Ligase III/LIG3 aa 800-850. The exact sequence is proprietary. (CAA59230.1).
Database link: P49916-3
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
This product was previously labelled as DNA Ligase III
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224820 was affinity purified using an epitope specific to DNA Ligase III/LIG3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 103 kDa.|
FunctionInteracts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Tissue specificityTestis, thymus, prostate and heart.
Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger.
- Information by UniProt
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
All lanes : Anti-DNA Ligase III/LIG3 antibody (ab224820) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
DNA Ligase III/LIG3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer) with ab224820 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224820 at 1 µg/ml.
Lane 1: ab224820 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab224820 has not yet been referenced specifically in any publications.