Overview

  • Product name

    Anti-DNA Ligase III/LIG3 antibody
    See all DNA Ligase III/LIG3 primary antibodies
  • Description

    Rabbit polyclonal to DNA Ligase III/LIG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 765-999 (internal sequence).
    Database link: NP_039269

  • Positive control

    • WB: A431, HeLa, HepG2, MOLT4, Raji cells IHC-P: H441 Xenograft
  • General notes

     This product was previously labelled as DNA Ligase III

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Tissue specificity

    Testis, thymus, prostate and heart.
  • Sequence similarities

    Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

Images

  • Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 113 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of Ligase III in paraformaldehyde fixed A431, using ab96576 at a 1/200 dilution.
    Lower image (2): merged with DNA probe.
  • Immunohistochemical analysis of Ligase III in paraffin embedded H441 Xenograft, using ab96576 at a 1/100 dilution.

References

ab96576 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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