Key features and details
- Rabbit polyclonal to DNA Ligase III/LIG3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DNA Ligase III/LIG3 antibody
See all DNA Ligase III/LIG3 primary antibodies
DescriptionRabbit polyclonal to DNA Ligase III/LIG3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 765-999 (internal sequence).
Database link: NP_039269
- WB: A431, HeLa, HepG2, MOLT4, Raji cells IHC-P: H441 Xenograft
This product was previously labelled as DNA Ligase III
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 113 kDa.|
|IHC-P||1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||1/100 - 1/200.|
FunctionInteracts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
Tissue specificityTestis, thymus, prostate and heart.
Sequence similaritiesBelongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger.
- Information by UniProt
- D11Wsu78e antibody
- DNA ligase antibody
- DNA ligase 3 antibody
Anti-DNA Ligase III/LIG3 antibody (ab96576) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 113 kDa
7.5% SDS PAGE
Immunofluorescence analysis of Ligase III in paraformaldehyde fixed A431, using ab96576 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
Immunohistochemical analysis of Ligase III in paraffin embedded H441 Xenograft, using ab96576 at a 1/100 dilution.
ab96576 has not yet been referenced specifically in any publications.