Overview

  • Product name

    Anti-DNA Ligase III/LIG3 antibody
    See all DNA Ligase III/LIG3 primary antibodies
  • Description

    Rabbit polyclonal to DNA Ligase III/LIG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human DNA Ligase III/LIG3 aa 116-403.
    Database link: NP_039269

  • Positive control

    • HepG2 whole cell lysate; A549 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.
  • General notes

     This product was previously labelled as DNA Ligase III

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function

    Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • Tissue specificity

    Testis, thymus, prostate and heart.
  • Sequence similarities

    Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

Images

  • Anti-DNA Ligase III/LIG3 antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 113 kDa



    7.5% SDS-PAGE

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab97404, at 1/100 dilution, staining DNA Ligase III/LIG3 in paraffin-embedded A549 xenograft by Immunohistochemistry.

References

ab97404 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting Abcam regarding ab97404.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions that may improve your results with this antibody.


I noticed you are using mouse samples in your experiments. Have you also tested a positive control from human? Since we have not tested this antibody with mouse we cannot guarantee that it is compatible with this species. You would need to test human samples to confirm the antibody is not working.


I would like to recommend the following modifications:


Block using 5% BSA rather than milk - we routinely test our products with this reagent. Additionally, use 3% BSA as the antibody diluent for both the primary and secondary antibodies. Continue with the primary antibody incubation overnight at 4oC. Also confirm that the secondary antibody is working well with other antibodies.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Heart, Lung and Kidney)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Brain, Heart, Lung and Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 16 2012

Answer

Thank you for your email.

I've just added the discount code toour internal notes of your review, so it is now active and can be redeemed for a free antibody.

Please let me know if there is anything else that we can do for you. Have a nice day!

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Answer

Thank you for your reply.

An account was automatically generated for your email addressin order for us to correspond with you. You can change your password and access this account by going to "sign in" onthe https://www.abcam.comwebpage, andselecting"Forgotten yourpassword?".

I have issued a discount code below, which can be redeemed for a free primary antibody such as ab125434 after submitting a review for ab97404. Please see below for more information and instructions, and let me know if there is anything else that we can do for you.

DISCOUNT CODE: ***
Expiration date: September 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab97404 in mouse tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us and for keeping us updated about the situation. I am sorry to hear that the suggestions from my colleague did not help improve the results.

At this time we have only tested ab97404 with human lysates, and though we predict cross-reactivity with mouse based on sequence homology, we are not able to guarantee the results in mouse. You can try the antibody in Western blot with human lysates, and if this also does not work I would be happy to send a replacement antibody.

For untested species (such as mouse in this case), we do have a testing discount program. We generally arrange this prior to purchase, but I will be happy to offer this program retroactively to you as well. You would simply need to submit an Abreview with the protocol and results seen with ab97404, and in exchange we will give you one free primary antibody. I would recommend trying ab125434, as this is the only DNA ligase III antibody that we have tested in WB with mouse lysates.

Please let me know if you would like to submit a review of ab97404 in exchange for a free primary antibody, and I will set that up for you.

If you have any questions or need anything else from us, please don't hesitate to ask.

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