Overview

  • Product name
    Anti-DNA Ligase IV antibody
    See all DNA Ligase IV primary antibodies
  • Description
    Rabbit polyclonal to DNA Ligase IV
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: IADIEHIEKD MKHQSFYIET KLDGERMQMH KDGDVYKYFS RNGYNYTDQF, corresponding to internal sequence amino acids 253-302 of DNA Ligase IV

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration. PubMed: 18451142
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificity
    Testis, thymus, prostate and heart.
  • Involvement in disease
    LIG4 syndrome
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation
  • Sequence similarities
    Belongs to the ATP-dependent DNA ligase family.
    Contains 2 BRCT domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA ligase IV ATP dependent antibody
    • DNA joinase antibody
    • DNA ligase 4 antibody
    • DNA ligase IV antibody
    • DNA repair enzyme antibody
    • DNLI4_HUMAN antibody
    • LIG 4 antibody
    • LIG4 antibody
    • LIG4S antibody
    • Ligase IV antibody
    • Ligase IV DNA ATP dependent antibody
    • Polydeoxyribonucleotide synthase [ATP] 4 antibody
    • Polydeoxyribonucleotide synthase 4 antibody
    • Polydeoxyribonucleotide synthase antibody
    • Polynucleotide ligase antibody
    • Sealase antibody
    see all

Images

  • Anti-DNA Ligase IV antibody (ab26039) at 1.25 µg/ml + HepG2 cell lysate

    Predicted band size: 109 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling DNA Ligase IV with ab26039 at a concentration of 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the pancreatic acinus. Magnification 400X.

References

This product has been referenced in:
  • Zhu Y  et al. Tamoxifen-resistant breast cancer cells are resistant to DNA-damaging chemotherapy because of upregulated BARD1 and BRCA1. Nat Commun 9:1595 (2018). Read more (PubMed: 29686231) »
  • Qiu Z  et al. Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break. Sci Rep 7:43156 (2017). Read more (PubMed: 28225067) »
See all 12 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us. Your credit note ID is ****. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for contacting us and for providing these details.

After review of your protocol I would like to offer to you either a free of charge replace of this product or another DNA Ligase IV antibody such ashttps://www.abcam.com/DNA-Ligase-IV-antibody-ab80514.html, or I may offer a refund/credit for this product.

Please let me now how you would like to proceed. Do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. I am sorry for the confusion with the last email. I mis-transcribed the abcam product number. This product is covered by our Abpromise guarantee.If you could send me the orginal Abcam confirmation number for purchase of ab26039 and some details of about the protocol and sample preparation you use, I would be very happy to send to you a free of charge replacement or to arrange a credit/refund as you are using it according to specifications listed on our datasheet. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. The data which I have seen for NUCB1, from SwissProthttp://www.uniprot.org/uniprot/Q02818the NUCB1 antibody datasheet from similar products offered by other companies and our own datasheet for ab26039 all point to a preeicted band between 54-70 kDa. I cannot explain why you are getting a 109kDA band but can suggest that the protein is not fully reduced and denatured in your samples. I would suggest using fresh protease inhibitors in your lysis buffer, using a sample buffer containing BME and heating your samples at 95C for 5-10 minutes. I hope you find this information helpful. If you have other questions please do not hesitate to contact us.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Vascular Smooth Muscle Cell)
Loading amount
25 µg
Specification
Vascular Smooth Muscle Cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Andrew Cobb

Verified customer

Submitted Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
100 µg
Specification
293T
Treatment
NCS 200ug/ul
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Maayan Salton

Verified customer

Submitted May 28 2010

Answer

Thank you for getting back in touch with me with your order details. I have arranged for a credit note to be raised by our accounts department against order numbers 126743 and 114423 for two vials of ab17988. They will be in touch with your purchasing agent shortly. With regards the antibodies against Artemis and DNA-Ligase IV (ab18319 and ab26039) I do not have details of your order previously. If you are referring to other commercially available antibodies against these targets then I must stress that the behaviour of one antibody is not necessary transferable to another. I would still like to recommend that these antibodies are applied as detailed using RIPA buffer extraction of the human cells that you have been using; verify the transfer of your protein by ponceau staining and use 3% BSA/TBS (0.1% tween 20) as a blocking agent. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. In Charlotte's absence I am dealing with her enquiries. I am sorry to hear that you have been having difficulties with these antibodies. I have been reading through your technical questionaires and I appreciate the detail that you have provided me with. I am unaware of the commercial extraction agents that you have been employing. However, I am certainly concerned about the extraneous bands that you have been observing when using our Werner's syndrome helicase WRN antibody (ab17988). I am certainly prepared to provide you with credit to the value of one vial of each of these antibodies provided that they have been purchased within the past 90 days. With respect to your observed absence of detection of Artemis and DNA Ligase IV using ab18319 and ab26039 respectively I would like to suggest that you use a RIPA buffer extraction of the human cells that you have been using; verify the transfer of your protein by ponceau staining and use 3% BSA/TBS (0.1% tween 20) as a blocking agent. My concern is that the commercially available extraction buffers and blocking buffers that you have been using are perhaps not the best agents for the detection of these proteins. It would therefore certainly help to prepare fresh buffers prior to an immunoblotting experiment. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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