Key features and details
- Rabbit polyclonal to DNA mismatch repair protein MutL
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-DNA mismatch repair protein MutL antibody
DescriptionRabbit polyclonal to DNA mismatch repair protein MutL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Escherichia coli
Recombinant full length protein corresponding to Escherichia coli DNA mismatch repair protein MutL aa 1-615.
MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDIDIERGG AKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALA SISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDL FYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYR AVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLTLRGWVADPNHTTPAL AEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVD VNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRS IPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQKQ QGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSAL EKAQSALAELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQS VFEPGNIAQWIARNLMSEHAQWSMAQAITLLADVERLCPQLVKTPPGGLL QSVDLHPAIKALKDE
Database link: P23367
- WB: Recombinant Escherichia coli DNA mismatch repair protein MutL protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab225917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
RelevanceThis protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.
- mutL antibody
All lanes : Anti-DNA mismatch repair protein MutL antibody (ab225917) at 1/500 dilution
Lane 1 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.2 µg
Lane 2 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.1 µg
Lane 3 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.05 µg
Lane 4 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.025 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225917 has not yet been referenced specifically in any publications.