Anti-DNA mismatch repair protein MutL antibody (ab225917)

Overview

  • Product name

    Anti-DNA mismatch repair protein MutL antibody
  • Description

    Rabbit polyclonal to DNA mismatch repair protein MutL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity


    Predicted to work with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to Escherichia coli DNA mismatch repair protein MutL aa 1-615.
    Sequence:

    MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDIDIERGG AKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALA SISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDL FYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYR AVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLTLRGWVADPNHTTPAL AEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVD VNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRS IPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQKQ QGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSAL EKAQSALAELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQS VFEPGNIAQWIARNLMSEHAQWSMAQAITLLADVERLCPQLVKTPPGGLL QSVDLHPAIKALKDE


    Database link: P23367

  • Positive control

    • WB: Recombinant Escherichia coli DNA mismatch repair protein MutL protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab225917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

  • Relevance

    This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.
  • Database links

    • Alternative names

      • mutL antibody

    Images

    • All lanes : Anti-DNA mismatch repair protein MutL antibody (ab225917) at 1/500 dilution

      Lane 1 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.2 µg
      Lane 2 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.1 µg
      Lane 3 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.05 µg
      Lane 4 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.025 µg

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa

    References

    ab225917 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab225917.
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