Anti-DNA mismatch repair protein MutL antibody (ab225917)
Key features and details
- Rabbit polyclonal to DNA mismatch repair protein MutL
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-DNA mismatch repair protein MutL antibody -
Description
Rabbit polyclonal to DNA mismatch repair protein MutL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli DNA mismatch repair protein MutL aa 1 to the C-terminus.
Database link: P23367 -
Positive control
- WB: Recombinant Escherichia coli DNA mismatch repair protein MutL protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
Target
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Relevance
This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA. -
Database links
- Entrez Gene: 948691 Escherichia coli
- SwissProt: P23367 Escherichia coli
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Alternative names
- mutL antibody
Images
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All lanes : Anti-DNA mismatch repair protein MutL antibody (ab225917) at 1/500 dilution
Lane 1 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.2 µg
Lane 2 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.1 µg
Lane 3 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.05 µg
Lane 4 : Recombinant Escherichia coli DNA mismatch repair protein MutL protein at 0.025 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225917 has not yet been referenced specifically in any publications.