Anti-DNA PKcs antibody [18-2] (ab44815)
Key features and details
- Mouse monoclonal [18-2] to DNA PKcs
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DNA PKcs antibody [18-2]
See all DNA PKcs primary antibodies -
Description
Mouse monoclonal [18-2] to DNA PKcs -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human DNA PKcs aa 1-2713. Immunogen is purified from HeLa cells.
Database link: P78527 -
Epitope
amino acids 1-2713 -
Positive control
- WB: Wildtype HAP1, SHSY5Y and K562 cell lysates. ICC/IF: Wildtype HAP1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
18-2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab44815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB | (1) |
1/200. Predicted molecular weight: 469 kDa.
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Notes |
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ICC/IF
1/100. |
WB
1/200. Predicted molecular weight: 469 kDa. |
Target
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Function
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Contains 3 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5591 Human
- Omim: 600899 Human
- SwissProt: P78527 Human
- Unigene: 491682 Human
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Alternative names
- DNA dependent protein kinase catalytic subunit antibody
- DNA PK catalytic subunit antibody
- DNA-dependent protein kinase catalytic subunit antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: DNA PKcs knockout HAP1 cell lysate (40 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: SHSY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab44815 observed at 460 kDa. Red - loading control, ab18251, observed at 52 kDa.ab44815 was shown to specifically react with DNA PKcs in iwld-type HAP1 cells. No band was observed when DNA PKcs knockout samples were examined. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab44815 and ab18251 (loading control to alpha tubulin) were diluted at 1/200 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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ab44815 staining DNA PKcs in wild-type HAP1 cells (top panel) and PRKDC knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab44815 at 1/100 dilution and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab44815 has been referenced in 9 publications.
- Wu WY et al. SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. Redox Biol 52:102303 (2022). PubMed: 35390676
- van de Kooij B et al. Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks. Nat Commun 13:5295 (2022). PubMed: 36075911
- Luo Y et al. Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway. Front Mol Biosci 8:652443 (2021). PubMed: 34746227
- Deshpande RA et al. DNA-dependent protein kinase promotes DNA end processing by MRN and CtIP. Sci Adv 6:eaay0922 (2020). PubMed: 31934630
- Hanson RL & Batchelor E Rucaparib Treatment Alters p53 Oscillations in Single Cells to Enhance DNA-Double-Strand-Break-Induced Cell Cycle Arrest. Cell Rep 33:108240 (2020). PubMed: 33053351
- Dunlop CR et al. Complete loss of ATM function augments replication catastrophe induced by ATR inhibition and gemcitabine in pancreatic cancer models. Br J Cancer 123:1424-1436 (2020). PubMed: 32741974
- Jang Y et al. Intrinsically disordered protein RBM14 plays a role in generation of RNA:DNA hybrids at double-strand break sites. Proc Natl Acad Sci U S A 117:5329-5338 (2020). PubMed: 32094185
- Yuan Y et al. Single-stranded DNA oligomers stimulate error-prone alternative repair of DNA double-strand breaks through hijacking Ku protein. Nucleic Acids Res 43:10264-76 (2015). PubMed: 26350212
- Neumayer G et al. Targeting protein for xenopus kinesin-like protein 2 (TPX2) regulates ?-histone 2AX (?-H2AX) levels upon ionizing radiation. J Biol Chem 287:42206-22 (2012). PubMed: 23045526